6ZG3

the structure of ECF PanT transporter in a complex with a nanobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F	Energy-coupling factor transporter ATP-binding protein EcfA1	polymer	300	33166.4	2	UniProt (Q1GBJ0) Pfam (PF00005) In PDB	Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002	ECF transporter A component EcfA1
2	B, G	Energy-coupling factor transporter ATP-binding protein EcfA2	polymer	287	31672.2	2	UniProt (Q1GBI9) Pfam (PF00005) In PDB	Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002	ECF transporter A component EcfA2
3	C, H	Conserved hypothetical membrane protein	polymer	215	23008.4	2	UniProt (Q1GBG0) Pfam (PF12822) In PDB	Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
4	D, I	Putative cobalt ABC transporter, permease protein	polymer	265	30290.3	2	UniProt (Q1GBI8) Pfam (PF02361) In PDB	Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
5	E, J	CA14381 nanobody	polymer	136	14979.5	2		Lama glama (Llama)
6	I, J, A, B, C...	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	10	Chemie (PEG)
7	I, C, D, G, H	DODECYL-BETA-D-MALTOSIDE	non-polymer		510.6	7	Chemie (LMT)
8	C, H	CITRIC ACID	non-polymer		192.1	2	Chemie (CIT)

Sequence modifications

A, F: 2 - 282 (UniProt: Q1GBJ0)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Gly -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag

C, H: 1 - 207 (UniProt: Q1GBG0)

PDB	External Database	Details
Trp 208	-	expression tag
Ser 209	-	expression tag
His 210	-	expression tag
Pro 211	-	expression tag
Gln 212	-	expression tag
Phe 213	-	expression tag
Glu 214	-	expression tag
Lys 215	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	266233.4
Non-Polymers*	Number of molecules	19
	Total formula weight	5019.8
All*	Total formula weight	271253.2

*Water molecules are not included.