6YXY
State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate
This is a non-PDB format compatible entry.
Summary for 6YXY
| Entry DOI | 10.2210/pdb6yxy/pdb |
| EMDB information | 11000 |
| Descriptor | bL28m, mt-LAF2, mt-LAF3, ... (90 entities in total) |
| Functional Keywords | mitoribosome, assembly, lsu, ribosome |
| Biological source | Trypanosoma brucei brucei More |
| Total number of polymer chains | 83 |
| Total formula weight | 3253211.74 |
| Authors | Jaskolowski, M.,Ramrath, D.J.F.,Bieri, P.,Niemann, M.,Mattei, S.,Calderaro, S.,Leibundgut, M.A.,Horn, E.K.,Boehringer, D.,Schneider, A.,Ban, N. (deposition date: 2020-05-04, release date: 2020-10-14, Last modification date: 2024-11-13) |
| Primary citation | Jaskolowski, M.,Ramrath, D.J.F.,Bieri, P.,Niemann, M.,Mattei, S.,Calderaro, S.,Leibundgut, M.,Horn, E.K.,Boehringer, D.,Schneider, A.,Ban, N. Structural Insights into the Mechanism of Mitoribosomal Large Subunit Biogenesis. Mol.Cell, 79:629-644.e4, 2020 Cited by PubMed Abstract: In contrast to the bacterial translation machinery, mitoribosomes and mitochondrial translation factors are highly divergent in terms of composition and architecture. There is increasing evidence that the biogenesis of mitoribosomes is an intricate pathway, involving many assembly factors. To better understand this process, we investigated native assembly intermediates of the mitoribosomal large subunit from the human parasite Trypanosoma brucei using cryo-electron microscopy. We identify 28 assembly factors, 6 of which are homologous to bacterial and eukaryotic ribosome assembly factors. They interact with the partially folded rRNA by specifically recognizing functionally important regions such as the peptidyltransferase center. The architectural and compositional comparison of the assembly intermediates indicates a stepwise modular assembly process, during which the rRNA folds toward its mature state. During the process, several conserved GTPases and a helicase form highly intertwined interaction networks that stabilize distinct assembly intermediates. The presented structures provide general insights into mitoribosomal maturation. PubMed: 32679035DOI: 10.1016/j.molcel.2020.06.030 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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