EMDB-11000: State B of the Trypanosoma brucei mitoribosomal large subunit ass...

Basic information

• Entry: Database: EMDB / ID: EMD-11000
• Title: State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate
• Map data: half2

Sample

• Complex: State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate
  • Protein or peptide: x 80 types
  • RNA: x 1 types
• Ligand: x 9 types

Function / homology

Function:

organellar large ribosomal subunit / pseudouridine synthesis / pseudouridine synthase activity / negative regulation of ribosome biogenesis / kinetoplast / RNA methyltransferase activity / nuclear lumen / mitochondrial large ribosomal subunit / ciliary plasm / mitochondrial ribosome / mitochondrial translation / post-transcriptional regulation of gene expression / cyclosporin A binding / ribosomal large subunit binding / : / acyl binding / acyl carrier activity / RNA processing / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / lipid metabolic process / fatty acid biosynthetic process / large ribosomal subunit / protein folding / large ribosomal subunit rRNA binding / RNA helicase activity / negative regulation of translation / rRNA binding / hydrolase activity / ribosome / structural constituent of ribosome / mitochondrial matrix / ribonucleoprotein complex / translation / GTPase activity / mRNA binding / GTP binding / nucleolus / mitochondrion / RNA binding / nucleoplasm / ATP binding / nucleus / metal ion binding / cytosol / cytoplasm

Domain/homology:

: / EngB-type guanine nucleotide-binding (G) domain profile. / Pseudouridine synthase, TruD / Pseudouridine synthase, TruD, catalytic domain / EngB-type guanine nucleotide-binding (G) domain / : / GTPase Der, C-terminal KH-domain-like / : / KH-domain-like of EngA bacterial GTPase enzymes, C-terminal / Pseudouridine synthase, RsuA/RluA-like / RNA pseudouridylate synthase / tRNA/rRNA methyltransferase, SpoU type / SpoU rRNA Methylase family / Fungal lipase-like domain / Lipase (class 3) / Protein Iojap/ribosomal silencing factor RsfS / Ribosomal silencing factor during starvation / Pseudouridine synthase, catalytic domain superfamily / GTP-binding protein, orthogonal bundle domain superfamily / Pentatricopeptide (PPR) repeat profile. / LIM domain / Zinc-binding domain present in Lin-11, Isl-1, Mec-3. / Zinc finger, LIM-type / LIM domain profile. / Pentatricopeptide repeat / DnaJ domain / Rhodanese-like domain superfamily / 39S ribosomal protein L43/54S ribosomal protein L51 / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / DnaJ molecular chaperone homology domain / dnaJ domain profile. / Chaperone J-domain superfamily / DnaJ domain / tRNA (guanine-N1-)-methyltransferase, N-terminal / Alpha/beta knot methyltransferases / 50S ribosome-binding GTPase / Cyclophilin-type peptidyl-prolyl cis-trans isomerase / RNA helicase, DEAD-box type, Q motif / DEAD-box RNA helicase Q motif profile. / GTP binding domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain profile. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain / Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD / Cyclophilin-like domain superfamily / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Acyl carrier protein (ACP) / Phosphopantetheine attachment site / Nucleotidyltransferase superfamily / DEAD/DEAH box helicase domain / DEAD/DEAH box helicase / Phosphopantetheine attachment site. / Phosphopantetheine attachment site / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11, RNA binding domain / Ribosomal protein L11/L12 / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / 50S ribosomal protein L30e-like / Helicase conserved C-terminal domain / Ribosomal protein L28/L24 / : / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal protein L24 / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / 50S ribosomal protein uL4 / K homology domain-like, alpha/beta / Small GTP-binding protein domain / helicase superfamily c-terminal domain / Tetratricopeptide-like helical domain superfamily / Ribosomal protein L13 / Ribosomal protein L13 / Ribosomal protein L13 superfamily / Prokaryotic membrane lipoprotein lipid attachment site profile. / Ribosomal protein L25/L23 / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A / Ribosomal protein L26/L24, KOW domain / Ribosomal protein L18e/L15P

Component:

Lipase (Class 3) / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Tetratricopeptide repeat / KOW domain-containing protein / T. brucei spp.-specific protein / T. brucei spp.-specific protein / Uncharacterized protein / Chaperone protein DNAj, putative / Large ribosomal subunit protein uL22c / Large ribosomal subunit protein bL21m / T. brucei spp.-specific protein / Uncharacterized protein / Uncharacterized protein / tRNA/rRNA methyltransferase SpoU type domain-containing protein / Mitochondrial RNA binding complex 1 subunit / Uncharacterized protein / T. brucei spp.-specific protein / Uncharacterized protein / Large ribosomal subunit protein uL4m / T. brucei spp.-specific protein / Uncharacterized protein / DEAD-box helicase, putative / EngB-type G domain-containing protein / Ribosomal protein S24/S35 mitochondrial conserved domain-containing protein / Large ribosomal subunit protein bL20c / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Peptidyl-prolyl cis-trans isomerase / TRUD domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein uL23m / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / tRNA/rRNA methyltransferase SpoU type domain-containing protein / GTP-binding protein, putative / Ribosomal protein L30 ferredoxin-like fold domain-containing protein / Large ribosomal subunit protein uL29m / Rhodanese domain-containing protein / Uncharacterized protein / Pseudouridylate synthase, putative / Uncharacterized protein / Uncharacterized protein / GTP-binding protein, putative / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Ribosomal protein L9 domain-containing protein / Uncharacterized protein / Peptidyl-prolyl cis-trans isomerase / Uncharacterized protein / Uncharacterized protein / Acyl carrier protein / 50S ribosomal protein L17, putative / Uncharacterized protein / Uncharacterized protein / 50S ribosomal protein L13, putative / Ribosomal protein L3 mitochondrial, putative / Large ribosomal subunit protein mL43 / Uncharacterized protein / Uncharacterized protein / LIM zinc-binding domain-containing protein / 39S ribosomal protein L28, mitochondrial / Uncharacterized protein / Large ribosomal subunit protein uL11m / Uncharacterized protein

• Biological species: Trypanosoma brucei brucei (eukaryote)
• Method: single particle reconstruction / cryo EM / Resolution: 3.1 Å
• Authors: Jaskolowski M / Ramrath DJF / Bieri P / Niemann M / Mattei S / Calderaro S / Leibundgut MA / Horn EK / Boehringer D / Schneider A / Ban N

Funding support

Switzerland, 1 items

Organization	Grant number	Country
Swiss National Science Foundation		Switzerland

• Citation: Journal: Mol Cell / Year: 2020; Title: Structural Insights into the Mechanism of Mitoribosomal Large Subunit Biogenesis.; Authors: Mateusz Jaskolowski / David J F Ramrath / Philipp Bieri / Moritz Niemann / Simone Mattei / Salvatore Calderaro / Marc Leibundgut / Elke K Horn / Daniel Boehringer / André Schneider / Nenad Ban / ; Abstract: In contrast to the bacterial translation machinery, mitoribosomes and mitochondrial translation factors are highly divergent in terms of composition and architecture. There is increasing evidence that the biogenesis of mitoribosomes is an intricate pathway, involving many assembly factors. To better understand this process, we investigated native assembly intermediates of the mitoribosomal large subunit from the human parasite Trypanosoma brucei using cryo-electron microscopy. We identify 28 assembly factors, 6 of which are homologous to bacterial and eukaryotic ribosome assembly factors. They interact with the partially folded rRNA by specifically recognizing functionally important regions such as the peptidyltransferase center. The architectural and compositional comparison of the assembly intermediates indicates a stepwise modular assembly process, during which the rRNA folds toward its mature state. During the process, several conserved GTPases and a helicase form highly intertwined interaction networks that stabilize distinct assembly intermediates. The presented structures provide general insights into mitoribosomal maturation.

History

Deposition	May 4, 2020	-
Header (metadata) release	Oct 14, 2020	-
Map release	Oct 14, 2020	-
Update	Oct 14, 2020	-
Current status	Oct 14, 2020	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_11000.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: half2
• Voxel size: X=Y=Z: 1.085 Å

Density

Contour Level	By AUTHOR: 0.025 / Movie #1: 0.025
Minimum - Maximum	-0.099372275 - 0.21032417
Average (Standard dev.)	0.0008963023 (±0.007486)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 434.0 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.085	1.085	1.085
M x/y/z	400	400	400
origin x/y/z	0.000	0.000	0.000
length x/y/z	434.000	434.000	434.000
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	400	400	400
D min/max/mean	-0.099	0.210	0.001

Supplemental data

Mask #1

• File: emd_11000_msk_1.map

Half map: half1

• File: emd_11000_half_map_1.map
• Annotation: half1

Half map: half1

• File: emd_11000_half_map_2.map
• Annotation: half1

Sample components

Entire : State B of the Trypanosoma brucei mitoribosomal large subunit ass...

• Entire: Name: State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate

Components

• Complex: State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate
  • Protein or peptide: bL28m
  • Protein or peptide: uL29m
  • Protein or peptide: uL30m
  • Protein or peptide: bL32m
  • Protein or peptide: bL35m
  • RNA: 12S ribosomal RNA
  • Protein or peptide: mL67
  • Protein or peptide: mt-EngA
  • Protein or peptide: mL68
  • Protein or peptide: mt-LAF2
  • Protein or peptide: mt-LAF3
  • Protein or peptide: mL70
  • Protein or peptide: mt-LAF4
  • Protein or peptide: uL3m
  • Protein or peptide: mL71
  • Protein or peptide: mt-LAF5
  • Protein or peptide: UNK
  • Protein or peptide: uL4m
  • Protein or peptide: mL72
  • Protein or peptide: mt-LAF6
  • Protein or peptide: mt-LAF7
  • Protein or peptide: mL74
  • Protein or peptide: mt-LAF8
  • Protein or peptide: bL9m
  • Protein or peptide: mL75
  • Protein or peptide: MALSU1
  • Protein or peptide: UNK
  • Protein or peptide: mL76
  • Protein or peptide: L0R8F8
  • Protein or peptide: uL11m
  • Protein or peptide: mL77
  • Protein or peptide: mt-ACP
  • Protein or peptide: UNK
  • Protein or peptide: mL78
  • Protein or peptide: mt-LAF12
  • Protein or peptide: UNK
  • Protein or peptide: Mtg1
  • Protein or peptide: UNK
  • Protein or peptide: uL13m
  • Protein or peptide: mL80
  • Protein or peptide: mt-LAF14
  • Protein or peptide: uL14m
  • Protein or peptide: mL81
  • Protein or peptide: mt-LAF15a
  • Protein or peptide: uL15m
  • Protein or peptide: mL83
  • Protein or peptide: mt-EngB
  • Protein or peptide: bL17m
  • Protein or peptide: mL84
  • Protein or peptide: mL85
  • Protein or peptide: mt-LAF18
  • Protein or peptide: bL19m
  • Protein or peptide: mL86
  • Protein or peptide: mt-LAF19
  • Protein or peptide: bL20m
  • Protein or peptide: mL87
  • Protein or peptide: mt-LAF20
  • Protein or peptide: bL21m
  • Protein or peptide: mL88
  • Protein or peptide: uL22m
  • Protein or peptide: mL89
  • Protein or peptide: uL23m
  • Protein or peptide: mL90
  • Protein or peptide: UNK
  • Protein or peptide: uL24m
  • Protein or peptide: mL92
  • Protein or peptide: mL93
  • Protein or peptide: mL94
  • Protein or peptide: mL95
  • Protein or peptide: mL41
  • Protein or peptide: mL42
  • Protein or peptide: mL98
  • Protein or peptide: mL43
  • Protein or peptide: mL99
  • Protein or peptide: mL100
  • Protein or peptide: mL101
  • Protein or peptide: mL49
  • Protein or peptide: mL52
  • Protein or peptide: mL53
  • Protein or peptide: mL63
  • Protein or peptide: mL64
• Ligand: ZINC ION
• Ligand: MAGNESIUM ION
• Ligand: SPERMIDINE
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: SODIUM ION
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE
• Ligand: NICOTINAMIDE-ADENINE-DINUCLEOTIDE
• Ligand: water

Supramolecule #1: State B of the Trypanosoma brucei mitoribosomal large subunit ass...

• Supramolecule: Name: State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#81
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote) / Strain: 427

Macromolecule #1: bL28m

• Macromolecule: Name: bL28m / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 28.051373 KDa

Sequence

String:

MLRNTDVRLF STFALNPETS VAPHGPPRGL VNRYVSMGLP PWAAWCNKVN RYSLYRMSGV TQRSFLPKPP QEMDVIWLNE RVRERVRTS RQVQNVYRQL KYPYVKTGIH YSDVLDHWVQ VPMVEAAMFE VEKDGGFDNF ILKRSGPELR STYGERIRRH I LVRQKEIQ KNFVLQKQAQ MLVESMEKEI LPMEDGKKVE EVLEKYGIDK EQLLRDIARA AVAKKQQLAS AVPQPSHVEV ST N

Macromolecule #2: uL29m

• Macromolecule: Name: uL29m / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 54.045367 KDa

Sequence

String:

MQRNLTKLSA AAFYEFVDNN FLNNKRPPVP GGSWTVEVLR NKSLADLQHI WFLLLKERNM LKSMKEHYLR HQEELGAMPA PSRLKMIDE SMRNIKRVVK ERDEEATARA VEIFKERLKR GIYRYPPGPP PPPGAHDKTS VVKVELSCYV EEERLRELFG R YDVFEPHK GIVRVELKLP DEVLKQKEEA EQLWTQYMAE CSDVKAYHQW STAAPSAYDY TEVELAPGIF ANDAISDKGG KH SGDTETH EGVIVAARVP VPPPKEKQPP PKNPLERLKA ERRSYLARTT IQLGYFPNVT LPPPRYETVE AVPRPVHPDE IEG PWEAYI TYDREDGLSY AQSLGITTIG VATVLGLTEH VREPQPYAVV DPVYCEALRR ERAREETLMK WPHVPEWKYE YSTY TRKHL ADIVQYNYTN VVDYVDREVL LTGKSVWECP IHIDHTCGGS KTVPPHAKKP VRYMDAGIAN VGVTDI

Macromolecule #3: uL30m

• Macromolecule: Name: uL30m / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 25.218359 KDa

Sequence

String:

MMRRMWVPTF SAQQQHLHHA MVLKMSTYAS TPSGNGVTSA ASNEEMNLHD AAAIAPGPYR RVGNIFIVHC DDHPFKHSWE VNRMLRELR LEFKGQTTIV PDIPQVRKRI WRVRHIVKVD VLDLDEAKAL IGVPEHISFT DLASQLPPSF GRVKAVPSPV I RSKMNFMK LRRMRLRDVL HRDALELRLL ELKRSAMKN(UNK) EQQKHAQKDE RTDNKEGEKC

Macromolecule #4: bL32m

• Macromolecule: Name: bL32m / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 9.74571 KDa

Sequence

String:

MFRRTFFTPM IAQPTLLMLG NKGGTPKRKK NPMQLRRKVY GLHFKEKYLK MEEWYYCPLC AEPKKPGEWC RREDCRQIKP

Macromolecule #5: bL35m

• Macromolecule: Name: bL35m / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 21.661453 KDa

Sequence

String:

MGSEESNNIC AYKRTISLAK IYIVLLVKTA MLRYSRLCFP KMGCEEITRK ARRVQLQPTE YLAQHRMQVW QLRFKEMGPP FSRVWVALG GKMRRRRVGR QVDVKDMRYY WRPIEPQYQR LYMSRLRIRD HSNKLRQPMR LRATNADIGS GSSSIEWERA S NRKYGAML APPKRQDFEF RVV

Macromolecule #7: mL67

• Macromolecule: Name: mL67 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 93.569273 KDa

Sequence

String:

MLRRFALTSS VALRLRFERD SGHNTVRYRP IPESMQPKHL EDNFTPFPLP KFDESLEYGP VRLRNIPDIE AAKERRRGSR LAATEVLLQ ETLQEENQFA TSGKGDGNMA IAITERHTED VTTPAADSRF PSQTMSPCSH EEEMRGYVVS RDYPLIDRLH C TRSIEELV AQFEDRPQIE SRVAALADMA STVSFRSDEE LLRMFTAISA PFSVDGRGLN FLTVKVSKFG RPYYVPNSLL PA YVNLVDA TTIALVREQP WRLSASPALF IQVLQFMALI KVFEPNKWFT FSDHAPSNRA DYRHAIGVNH STAFWGTGEE LYD FMVELL RVEDDGRIPT MLDLCTREQM VDLLSGFCGV MPCGKAVGDV FKTITDAFLR RVRNDISGPW SAHDWAIVER MYLV TVLCD AGNNEILQLL LSDTASPRGP DFFAAVSRTK DTPTKKRALC LLQEAIDNAS AKADKVTLLG LLESGSEFLL SLVDK GVAH TFATQNLFDY RILNSFLHCS LVADRLRVEQ SVITSLIPSS LRDVQVQMLM SNERNALNPL TSSLPGNSGA IATAPK LKR PLMTMLSQLE YLNSIDSVFI LHSSLMATST DQLVSAVRRL PSGKDSLIVT MSCLRALSVK SLTSPSMKER IACARAL EI VSYELEKGRA VLLPFSEEIL LHDAGAYCDE DLMLWTVAAF LARELPLVKV HTLMHSNCTA RTPYRFLKGG HNLLVSSR S LYDKGAPLLS SLHSKELRLV THNVRLRTPV RDRKCTLQYY NPIRARFVYR RDKPLFDKYH VTARNLAPGF SRGALKHDW RALGVYTPDH PQVPYHPLQT WMLGETTVEA E

Macromolecule #8: mt-EngA

• Macromolecule: Name: mt-EngA / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 65.528254 KDa

Sequence

String:

MRRCIPASVL R(UNK)VLLLQTRL VSTNTQTPPS GCATKIQNES PMNGSFNLPR ERQILGGLHA DWRTQMKRSG SILNDI TPA LPDTKRTEEQ RRRVAGWRPV VKLLGDQRLR IAIVGRMNSG KSSLFNLLRL EPTVPGRSNV VRDFDGITRD SVEGQAQ LE GMHFTIIDTP GMVQGRMVEE AFRTVETADA AIFVTAVDED IMPEELSLMQ YLHLKHMPVV LLANKMDLIQ EEEEEAVL D RYNSLGFGNA IPFSARRKSG LEMLAAVLEP LYHIHAMHKV ENDWDIEDLA MQGDESAMEE IRERNCSDRF IRIAIVGRT NSGKSSLVNR LVGFERNRAV DEKNSTRDPV ELPCSYKGRK LKLIDTAGLA RHRYRADRDF IGRIHGLSVN EIRFAHVVIV VFDATEGHP NKYDMAVLHS VAAEGRPFLL CANKWDAVLD QSATAEAIDF KIKRQVREVK YSNAVVVSAH TGLNLTLLMD Q ALELYDKW NKRVRRAELT RLWRKMEKSV IIPYHVARIG RITQVNTRPP TFLLQLQTKN DSNTLPKALQ EMMKNTLVEE FD FRGVPIR LIQEVKDSNP DYI

Macromolecule #9: mL68

• Macromolecule: Name: mL68 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 62.806293 KDa

Sequence

String:

MLYTRRLMTT GGSATADGAV SYSKGSYHIV PKKYTVGKRI AVRSYLDRNR TELSDRTYMP QKAWFEPYTP KKFDMEHQRI SHNFYNLET KLIWTAFDTP ELIGILLHDE TIKGAPHLYD AEFLESAVHW TRESRYWRCI GITKPFYNKT TLRAQCWHDR G LQVGTLVF SQAMRDALMD LERAVRRKEL GLEPNYVWDR WGPVGFIDGA RTDHLPRFAH NPYVDPDGVE VTEVDIAPFN TH EQIKERY GAFIDPDLRP FEGVFRAPSH GALTLDDVPH QEAVRLYRDL MEKADMPVML GNGAEIPPMD MRALFHLSAN PER MKAASE LSSWREVRGM LAPVQEVCDE KVEALRLMEN TRHDAARVRT FYEEKCGFSD FMRTPDKVIT AAVLCYLQEL QRIC TETDW GKPLARCLTD LERVNVMGKD AFLVYRHIED AILDKKRRVW ATRFAGEANE ESTLDYLLEN FGRRTEQTRN VGTTG TEFD REQEPIGRQV QRRVLDSDKA SKLAEVRQKR GKMWSNKKSV FDSLHQKQLQ NVTYGVH

Macromolecule #10: mt-LAF2

• Macromolecule: Name: mt-LAF2 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 85.715258 KDa

Sequence

String:

MGRRNIVLLH PTAHWTRARR SFVRSVALTT LLNCSGALLT TSRGIIVPQQ LKAYGGTFSR SVGERSNHLV SDVVLQSLSP SSTHNSPLL SKNIKTKTVT SFEDMPLYPE LRSALRQLRI TTPSPIQQSA VEVIMQRKDT VVAAPHGEGK TLAYLLPLYQ N MIKDRDVY KIPLRERRPR MILLAPTREL IAQLHHVCSV LGEATGLHAI SFTSRKRANH RLSRLLKQQL VDVMIMDPKI VL RLIRARR VFLDDLRYVA VDEADGMLSS QHDHDAVHLL MKVQKRVMFK HLWPVQTQTV FATAFITRKL EFVVGKKYPD AVT CIRRQR MHRPPGTLRH RFLPVERERE KMDTLQHLLK KHGNRPREIL TDIEEEQAYS KPHYLSGSLE ELVELRGLHL RRRP RMERH DHEGETPIAT LSSTSAGDVS NYKVEENRSG FAAKGDNADE MKERVRHALE GVRPVHWEHL TTVAAPFTCH VPRTV FADG KRCIIFTRGI DCATAVYHRL RGCGYTCSLL HAALPAAVRR RMFADFASGR TNILCSTDVA ARGLDLHVDF VVNFDM PTN ALTYLSRAGR TARQGRLGQV YSLYTKRQGV IVSAVRAFLQ KQLPLEGISN WKRHMMEPRY AEWRTHKLNA IARSYVS LI TQKTIPAHLE RTYLRHNATW RPLFHPQTTG VHGGVPPRQQ QRVMDAITWK AVCFRRGLLA RRKGGRAKFG TRAAESGV W NDVGGITSRV VANMEQNPHP GGDDFGPPRG PPK

Macromolecule #11: mt-LAF3

• Macromolecule: Name: mt-LAF3 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 46.374652 KDa

Sequence

String:

MLHAVRLLSC WRPAAIQHLP DVAP(UNK)APVQE VEKSHARYRP LSLRARQTLS EKSDDLQYHV VTQEWFGERV DSFLTC HYP QWDYETIKRL VQQGHIYRYR KNGKKKFTRL TDRLEFDELL VVPTRAFWEK QLAPPSGVLE ETDGPKFKLS ATAREMA HN MVLFKNEHVI VINKPHGLPM MPTDDPQEMS IAAMLPAWKF TNVAKPVVCH NLDRETSGCV VLARTRNAHR MLGRMFVK R VVPNSVYWSF CVGKPTVNYG RVRMHFDITR GNKGDIIVAR PSPTKTSKVA IAEFVVNASA LEFGSFISFY PLTTRRHQE RIMAAHALRC PVLGDAKYGG DAAFPSSLSL FWDPENKGLP LHLHHRKIQL PYKNTAGEFI CVTAPLPTQM EKTFKKLGWP CEVDDPLIP G

Macromolecule #12: mL70

• Macromolecule: Name: mL70 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 62.44241 KDa

Sequence

String:

MCLKRKAPHL FCFCLWSIFL SFRCFCFRSY AIMLPLLSFP TIQISIFLSF KLPITTFLLS PCFVFVFVFA IRYCGELTLN AQLVLFLLY HCAQTQRGPL KEGEMPICPG LCGELAAVPF RVFLGTLPTL AVEERFLRQL QPVFAWYSSR KRVKEQANEF I EIDLASCD AELLLRYSHI YYVRRQLFDE LIERQMTLLD SGKAPKMAEP SLLQCLAGCN MTIADRLQLE IRQLGAAKRA AS VPGRREL DPVARLEVYD YACMMRLVEE DAGAVGDAEM KARAYLPREV IESKLGHLTQ LLLGSDARAA LDKKDVKLLN RMI PPDYTR VGCVEKLRPF DVTAYFRFYG ERINNVKVEN YFKRALWGHV YRRFATTPSF LSGVSTYWAR HSGLDASFTT TTMP QEVAV AVCDQQIQFP AIKFRAQYVY TSPETARQLW RTDAAVPLMR LFPLMGSRTA EDLAAGVLTD AFWMHLGLSE EENLL QDSL LLKVRRFVDE VGDMYETNID SVLKRVDDNF KQVVPQLKAE DLQVDAPLQD GEGEDTVRET VAA

Macromolecule #13: mt-LAF4

• Macromolecule: Name: mt-LAF4 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 69.460422 KDa

Sequence

String:

MKVSSRGPIV RWALSRPTSG LVSSPSEWRC GRDLSDEEKH SGLLLRIRDI CQPLAKNDQL PVAVVKAQPS DLQVNEVDAV GDVAALSKV VKGKWRKISR QKTLLIEDDA RTPFSDPSKS FSPRVQSYGE YVRLTGKLPR DLPVLRFVLY RDSYSLNSVE N RLGYVLSL QPDCVFLRDQ PGGSFGCITQ HGVCLGVTKE ILSHASRHYN LHPLIFEPRE YFSTDKLHSL LQGARGHHHR VL LRCVEGS QDTIRALLKK TAERGFINYF WLDRFSVGTN RFFDMAVLAA RGDYLKSIGA LLHCVAESNG VHYDHFLKYL NAD PSTVP(UNK) IAQTWATTAK HMRSPNWIVQ LLRGLHKYHA DAECGKSSYL AELWSALPMR EALRRSAAEF VWNAMASQRL LSKGLNVVE GDVVRMGPAD SVSGCDSSSL PNNCGNYHLV TKDDEEKGTF KITDVVLPVP YGSVAANNCL FPHLSPLDKK L YVEFATKH GMSFLFDEQM PSPLSNPLQF YRHLITKPVN MQVSVIRDPN SLTSIKSDLA VMQERKLVQI GDIDYSTRVR EP CVYNVSE RFTEKMEEIL KTHRGPNSVV LSCYLPEDSS PFVMLREVFD LRHASFHDLY GLL

Macromolecule #14: uL3m

• Macromolecule: Name: uL3m / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 55.073699 KDa

Sequence

String:

MRSLIAMRRR FVSGTWTIAV STQQRHRSGS GPGDKRIRTD WYRCYPSLMR EKDRDMYHCY YPYLFDHGDK MSLYPKIPEN PREWQPEQL QTTYDAIRED KYDAFIRLRE KFPELYQDTR AWDNPPPFGE FNMFYSVRFG MVGVKAFTCK DYDELGNQFD C TAFWFPDN QVVKHSTRNG EVGTDKVYVG AMNVPVEFHK PHVAAFYKAA GVPVKHVCAG FPITPDAYAP VGTKLDVRHF KP GQEVTIT FQNTDYGFRG VMFRHGFDGG YVWLGDSRWQ RRPGAMGTEG QKRIYPGHRM AGQTGAAAET YQGVPVWRID YKN SLIYLP TLLDADVGTY VRFSDTINTK GLTLWNEHRG LPAFPTFIPP EDEDLSKLAT DECQLKSPPL YMYFRDEFPA TQLV SQADV EDAKSAKPAT APPKKKVYDM KKYYEARKKY RQSMQKARKY KLMGLRTKAH EKQEEARRAK ILKYKRMK

Macromolecule #15: mL71

• Macromolecule: Name: mL71 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 50.313797 KDa

Sequence

String:

MLFRSVSCKN YQRGGWSPGS KHQKHMTLNP TLYLYRFPGP HGPGPYTMKY WWTLGCFPTG MEVPFRLHEF LSTYQQEHVP VEVEEWLRC YIKDPLSELV NASNDFFKAV EVYPEVESAR GYKTLQPSIA PLLVPMKKFE EQLGVKISPV GLRSVLSNPV L KDRFLDDL FDYKSYVEKG GSTPHRRLAR SRFEGSLSVL GECEKCLPEQ HQVEISESLG TFIGATVSPA ETTADDERSL IL LLTTISE GCINAGNYSD AASVLADALM FCHDPDSQAT THANISFASL LNADFKGAEY NGREAALLQP QVKPTSTACA RGY VGWAAA AAYQDDFEKA EAIVKDGLTL YVGNEHLEKL ANKLQALREE QPSVYKQVPR SLRESRSHLP SQQSRGLLSG SGKG FSNEF DWVEFKNKLY PSKMDPRNNE MGSVFRRVGD LGSFISTSRS MERL

Macromolecule #16: mt-LAF5

• Macromolecule: Name: mt-LAF5 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 67.167289 KDa

Sequence

String:

MHWPHGKCGI VLTVRLPLLV VDAMPRRNFL RAPLSHSFMH IGFAIMSQRR WQSTIGEQRP TARRSKNAYS HPLAPKDPAS RNIVVPDAV HAKVGSPDTL PAEILQKRAQ RALLMQLQQN VIWEGTVISD ERHHLIQHFV KLRQNPKYRS SKQMMVVGGR H LLHELHKR GYTPRHLLVR EGQQKPKWAT NTGVKTEIIR VDRHVADVCS PGNDGFIGDF DIPKPPPKES LIANKQRFDR VL VLDNVDD PGLLGTVLRT AAGFHYDAVI ATNHCADLYD HRVIRAARGA HFQKAVPIYT LKEEDGDNVY GMLNHILQRN DLS PVCFAA RDDNDATDEL DDLVRQLRSG CAQPKSRTSP CGSPAVKRET LSDYCRNNFT KSDAKGQLLM VGPNHKRNSV RRWS KQLSI PVTQLLLDEV SQTDALIAFS VVLHALRPHG NWDYLPLHNN QEQQETASLE LQGMKASVDI GPNRFDLNEK DLSLD EEEQ VEKARLDNEL MRWRRLQRAQ GSDYDHWMEA ETRRIQEMAS NESRKQVSPW ARLRTRWQKG AVGMPPWVPN IVDEYR TPL DRDALRVERE VSESHVRPEN YDR

Macromolecule #17: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 2.826475 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)

Macromolecule #18: uL4m

• Macromolecule: Name: uL4m / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 53.156664 KDa

Sequence

String:

MRRVSFSVVL ATFGKRHSSI FSPRYDWRTS GVHDIAPRDE GDFLYQGPQH VLPGAHPLPL HHPHNTITRP VISPYIPSPQ RSHPYFTAP LPELPHFSTT KPIVYTYGTM KERIIAPVFN LKNEVIYTRE LDPFIFGMYP EVEELSKNLT YWMVRCQNFA S KWDYETRE IWRKAKKNWP NTGMGMPRVG NRKNHLYTWG GRTKPSKPWN MLMPTMDVKT WSKSNRMMLT LKMLQGRLQV VD RLTLEEP TQECYLELCR NMSWDVRHTG GGVLFMDGGS RITPSSEFDR AFFFGSFFNG RNKIVRPTVL CDEQYDYNKT AAK QRMKGP KGAKNPIPIN RFNAYDAMKH DRLVITEGAL MQLEDELYEH KLQILPPHIR NQLPEYGYLD SEALGDCVPS LKTI QMEAA ARTEEAESDM YKSFIDNPYN PWKDNMDASY AVDGADGTVQ KFVDGKKVSW SMLS

Macromolecule #19: mL72

• Macromolecule: Name: mL72 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 49.407 KDa

Sequence

String:

MSRSAFSSRG ALLFSSAPLQ CQRRWVTRLY TSYYTGVLYP NQLVQPKQRL PADVSVSAIL QKRSEPEAAA ERGSNKGDKV DVSKGEKTP RVSWDDADST DVMRVDNTQQ LKEAPQVERP YVPLGEVAKL ELQGDYYMEG GMFQEALEHY GVVAKAYNYA Y PENHAQRI GIRIKLSAAF RQTGRLESSL ANIEEVLRML DASTRPSLEL ICEALLELGI TREALGMKRE ATEAYEEALE VV NSFHNWG ESHRMLRLLP RLGRRFNYNF EEKFVYFSPF DYDRTFALVD QCLERAETIF NEIGDVEGAI RVLQQRKEMI DKK FFNMRD FAGRIHTMRG HWKRRAQHLT NAPTPDELLR YSPTIHQVHR DFKYELTAPI GREKEVMPGV NRLVLDMGNP YRRR GRLSN KMLKDADHKF ANYVRQKEYN E

Macromolecule #20: mt-LAF6

• Macromolecule: Name: mt-LAF6 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 40.106055 KDa

Sequence

String:

MNTVKVLLRK RVVRAGAFED TALALTNARS AGAEAKGRPI VLQSLD(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)PTIS FSG RQLWNSTKLL RENGNVRRSH GACVVGGASA IRRVWRDYKI RPNVVYVPDT EPTVASWCLE DELPTCIVRC SPVEINR GL LSAELADGHA AEFPIPASPS VETFLGEGKP SRLTSMLVLV GLRIPSNVGT LIRAAVEMGF ESVLLINCLD PFGEKALR A SEGTVFSPQF KIFEPGSDPV SALNSIAVEH NLLPLLALPS QKAETAFEVA KNLHKINAMR RSQEAEGDAA ASHNHIGPL LILGSEAKGL RDLAGEWSVP RKFVSVPLPN STVESLNVSV AGSILIHAFR PAAEKHFVEL EESARPRTLG DGPVLLR

Macromolecule #21: mt-LAF7

• Macromolecule: Name: mt-LAF7 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 18.645312 KDa

Sequence

String:

MPNIKGGVGS FLMRRAAPKS IRQKYQTGPQ FYKRKFFQFQ KGHHRLHRRI SGVQTGSPTH QREYERFHHL PGDVRTRPQF DFTFGETRA DRVMFAWRKR GDLQLYQMSG RGETFVCYRC GYPVRSQLVA VKADNWDYRM CYRCYTNTVH RGMENDT

Macromolecule #22: mL74

• Macromolecule: Name: mL74 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 39.765176 KDa

Sequence

String:

MLVPGLSLTR RAVTSSCCRP LHVVRGFSTT CTLFGLEQLQ DVPTSTSRRP TGLHRGPGKR QTSEREAAQY KFIRRWELQM RDEWDQLEP FKGLPKPKRQ FGNEAAEVIW PYALLLERVV KVHPFTKSIY VYYAQRQSTA RGKLAAEIAR SFAREFLIPI T FHNSQVYT EAEMLLEYSE TPWVVLHSLD NGQKPRILPV APVEGTPAHT AVEQLLAEVV QGCEALGASV ADPVTATRVL NE RPLQNQY VRVDYQWFGD TPDERASHLV RWEFEPEQIE PKIRHRTRHV LDWLNYDGNL PTHRAVHVNA MREKARQKAP RTV AGPRTF YNSAGSRANA RSSRFGGQAA VGK

Macromolecule #23: mt-LAF8

• Macromolecule: Name: mt-LAF8 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 68.904961 KDa

Sequence

String:

MKSVFDIARS HVTFPVSRDG TALRRVLKDW LDYTECQSLQ AKPAFPAELC ITVHPSVKSM SRVYTQGDPK MSGEVCRRPG EAASLSYTK RVRLLLWSAV LPWEVQRGLS MSVLEPPGNG GSVLGEVGVR HGLGGSGGAV DGGSAVATKG WKEVEDSLGV V TDPTT(UNK)TA ADFVSGPQCS TLGASIIPGM LFVMSPESAA QGLCFWSGAI RQPIDIAFIA PVEPPAADTP SFSELRQR R LQLSLEGYDI SRFFPDGELE SPTVTFAVQS HSYLDPFPDC EQRDQQVGCG SSRERNKGIE DSRRYTATPE GVGRGNENV RYVLETRRNL LRDSIRSALR ECRCTHGGVV WASNTGCAAD GNPTGTGECD VEVTISLTLS DELKEDLREK ARLYTNYVVP LEGHVRRHI KCLSGISPHP KGDATDGSDA LQQKGTEAVW GEEGCVCTNG SAPFVAPPPI IKPPLPVKVG TLASTRPRSP M LADEAEGR PTRLAPSVFG RHDAPALQRA QQECNQLISA SALARIPNTS PRAPEIPPID YEIFDLCLRL GLCQSEAIYY FY GRIMREW SKELRRLRAA KSHGEGGVND GNDMVLREED VHRMLRLVHD PSLQVPPELS ACVEAVASLR KITNEVGVPV V

Macromolecule #24: bL9m

• Macromolecule: Name: bL9m / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 30.729322 KDa

Sequence

String:

MRRTVVQRVA PWVPPPRHDI KVTMPPPPGG EVGGRFGVSQ GYSDRLARTP YWKRMALSTY KLRMMENATR YPMSEHRPGE YDIRYLPTP YPCTIRNRPL LEVGEPRQIP SIRIPVIFLV NLFDEAKGCW FGRRYETVYV ERQFMREELM PQRYAIYATP E AYKLLGLP VVNHHTHEEI PKTPREYEKL LERQRYDEER WKYTIEYLFR KYEDGPPELL DRPEDGWDGS EEIALSSVAG AG RDGVVSQ R(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)KIKLF

Macromolecule #25: mL75

• Macromolecule: Name: mL75 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 39.770539 KDa

Sequence

String:

MLRRSDLLLK KGWTHNPGRT RRGGKNLAWR PKMSERTLEQ FVPLHLAFPR RHPNSWQERQ FHLLGYVKWP KEIGFYNAGD NFELTPQAA YRIYKQNCDE TFWTRLHNEK TIIHLLPLVE QDPGTNMVLV DDIFRHHLKR FGADHYIYNA VMQAAAFAKD F PRCEQLLA EMRGLGLEPN AQSYVNMMLG ARLTGKPRDQ AEAFFREGIK TGAISAVMRL DTEFQMWMNQ LERLGSFKAK VG YLSVNEE GASPMPRDMW ALWGWHRTEA KFISRKQMIS EQVQNRVRSG KELVGTVYQK ARRQPWAKYN GMFPYDYNGP ARR PAASFV DAPTPTHNAE VCGTAY

Macromolecule #26: MALSU1

• Macromolecule: Name: MALSU1 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 39.553547 KDa

Sequence

String:

MRRIFSKSTP GYYARGVFQT TPSFRWCCFE LIRESGRMRN RLCCHLPLKA ARWHSSDAFT NDGTPPPLPP SNGGESDFVD LQLDDLTNA LGSASARREV GDSSELGVVR ENDIPEGQVF SRLNIPISSH QEEQRTNSRS ALRVSASDTL EETIQQPTST G SKQPLPYD DERFADMLLE MEVEGSLDET WQDGRKAYIE DVKEILEVLR SLKVRDICAI DVSNKTSNFD YMLFGTCEGP RH IHLAAWA VQEADALKRV CKIRRKQVDH TWEVVPVGRI IVNLMQESLR EELSLERKWA VTKCMDPLSV ANAPVSEGRS VKA HGLWTL TLNLQDLEDF EVDYCKDVLL TQV

Macromolecule #27: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 1.975426 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

Macromolecule #28: mL76

• Macromolecule: Name: mL76 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 39.638914 KDa

Sequence

String:

MLRLSSWNLK SQHHNVLRRS RPHIHKYREL NRWQRQAQGI SKWDQSHSHR PLPYVERFNP ESVGLTRGTS AFAWKWWHTQ YPWLPNVPP EAAQIDEAQK QERRSHRPPA WDDEFAKVVL NMNDAEIREY LMSKLTDVIF LETQRDGYEL RRLDFEGKPL T SLPEPRII ENFVLEEETI RERVIYQVVE GVFRLSPTSA DRRELRSVAN IIDYVLTHVR AARPTDRERR QERPITSAAL AV MQKCPIQ PQLGFVHALP HDTRDALLQE WERMHHLDWQ FGKAVYTPRS KENVRGNLTW LREDRHYDQR MKFMQEVESG EAR AKHMKL IAEAAGN

Macromolecule #29: L0R8F8

• Macromolecule: Name: L0R8F8 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 13.444574 KDa

Sequence

String:

MLRSTAACLG GQRKWWKEGR PDFGRAAARR MQLERRRLEA STHPPPVDPT AQQACVLYRR LLKAGHQQLL VTDKSYYARK VRHEFEVTA RQTSARVRGI MYERGQWLVE NKLGGIV

Macromolecule #30: uL11m

• Macromolecule: Name: uL11m / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 40.266777 KDa

Sequence

String:

MLRACGVLQL RARPKTVCVE PGSNRLPEAL VVEKARDIFG RPEFPGKRVL HNWRFFIKAG KAATGPPVGQ EFSKLGLKAM DFAKVFNDR TKPHFKEDVE LIVRIQVYFD KSYLFTIEPP PTAWFILRAL RKKRRETGPV PLRGHYCALM TLEMAYEIAK M KPLCWGRP EYPLLETRVR RVVGQARRMG VCFIGVDTPY SSPVKDMTEQ QYTEECERYR RIHMEQYTTL RQRELEEAPL IE RLHRPNM SPLTDEQIEE GLRDPCLLDT LWRASHPLSP YHRDLREREL ARRYLNARGW VKDMTPEEMR IVFMNYRLPE GEK RKQMDE (UNK)AMSGEVYWT RDGAQL

Macromolecule #31: mL77

• Macromolecule: Name: mL77 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 43.754648 KDa

Sequence

String:

MYVFRFHISS FHSFLVNFLQ PSRGVGEPSR LL(UNK)HLETHPS YMRRVTSTAV HRTVSSVLS(UNK) ALLPSFNGTL LL SVCFASC SAAPVRNLYK RLGLDHKATS EEVKAAYRQR ALECHPDVVD DNQKAQAEVD FRAVSEAYDV LIDPQKRKEH DKA LGLERT VPPAKKQQEG EGVGCDSKGS FNRGVTRPRN RKPFVRGDAD RNFREAFHGM SLDQVLFRER LRQRRMQKQM EEKA KEGDD GSPAGREESI RRVAAAAAER FAEKVRRQYG PGMLRHARVY TSLSRDPQPP PSDYMPFRPF HGWTVPNGVR TPPEP TLGP TAKVEDVKDV QLAEPAVGDA SHQRKLPKHF PVVQASDGSS LLREETIACM ERERRLPHNM GKLYSYHRPY

Macromolecule #32: mt-ACP

• Macromolecule: Name: mt-ACP / type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 16.550807 KDa

Sequence

String:

MQRSQLARKF ATQVGAYSLL RGRCHAVSTW KVMATQAVTI PMYLPVRFHS EGHAAGEEAG RTGQYLLNKD DVLTRVLEVV KNFEKVDAS KVTPQSHFVN DLGLNSLDVV EVVFAIEQEF ILDIPDHDAE KIQSITDAVE YISQNPMAK

Macromolecule #33: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 2.145636 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #34: mL78

• Macromolecule: Name: mL78 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 35.104535 KDa

Sequence

String:

MGASAGLIRR GGGVFPDAVS LTLTPSRRVY GGSGRGDLLY ENPDARRHSG RALGVLNGVR HSSQATMPES GQLYYRKLIL HSRPPNGSC AGLQRHCHDT CNWSYLIPSL HRCAESAISA KLWEKMCQLG LEDRSKAWVN LTQYERQRVR DGQNLYRYEV H QRLPLLEE SIGWAQLDDL LGWFRSARRA WVRLPTSVTL SRESSEAGVA SVVSPSSAMS CRLEGHADSR DTTPGRNQVF DT PERVEQL TEATVHRIRE ELQRLNRSER SDCEGSAAMR ASARRLARDE ELSRCVEEEL GWHGVALQHR IPVPK

Macromolecule #35: mt-LAF12

• Macromolecule: Name: mt-LAF12 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 77.908438 KDa

Sequence

String:

MRRLFITTAS TLCHSLHCTD TRTGGAGKES TPTEVQCEMT LQCSDESGCS PFLSSLLSPV ETVPLHDVTR TYSTMDVVDP PARYNPMVP NVEPSSSSAG HMEQ(UNK)LENEE EEGPVACAHK NGKLWGVFEG SEDNKPPAWF YRLCKDLFYR TNSEDNM DD AALVSDIEPS HYISSTENRH VDGSDTTQRS AEAGTDVSDG VDPYVWIPFN LLDEADYHVG PYRFPSTATY THEQRTLL C LGDTRREYVH FCDSYAFPGR AQIPTSVGTC PSKLYVNPKQ QQPVVYIQLS NDIPPAMWLP VKGTAASVRR VLAEFASMA ALHRDWHHDE FMERHATAVR MLELQRLPAG EGDILRYMAY DARNAQFAFA PIREFPNQQE FFLGEHDDPE KLMEHVDLCP LLFAIPHMR TVVDLHAEHM IPTIAGPGVA TSLYRCIYSK ALLFVQVHLS SEVKLPPQDP EAFKFMWKDS QVLPKMRIPV F VRVVWPTN ERMSGGGGLL RRFNRLFGTE FASDIPVDAA MALLYVMQWS GHIKDFLGVR GMRQRLADLL LASQQPEPTK LY PGTREIP NPEYTVAERL GMHVQYLAQL HDPDISLTIQ RLLPVASAPV RMGCAKAALI AGDRELFRHI VSSEPPGRMQ TYM TKLVRK RKTRDLVDAE PRLLEDQYEF AAPLWTKRGK RLDSNTLEGV VEAQSRLSG

Macromolecule #36: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 6.145567 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #37: Mtg1

• Macromolecule: Name: Mtg1 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 50.416492 KDa

Sequence

String:

MRRGLTCRNV HDPVFMRNVK RVLASYKESM DHSSRKGMSR EAFVDINEKG AAWYLGHMQL ASRTLAEKVK DADFVLEIRD ARLPFTTGN PNLQKIIIDR PRLIVFNKAE MSNEDCNRVI QQYYERTGNF ALFTSAKRSW RDTVEAVQRF VTHILPAQRF K TTANVGLV VGMPNVGKST LINSLRLAHE YQFHREDFRR PRTPEAVSIA PGTTRGVKLV PVCKDPNIVL YDSPGLTLPG CF AKEAGLK LAACGIIPTN DITLPRSLVA RYIYDVLSAA GVGEHMAECL HLPRAPISFD DCISMICERS GTSGQTDLGN LDP SRAQKF LIHDFQLGNL GRITLDKLPN KVRQAVSGKE YQRIGGGAGS GTEASNEENE VVWTHDVKSS DVVARYSEEM REVM EELQG E(UNK)VENTPSNR KPCGDSTVIS RKKGPISRVS AHDESFRRSI RIIPGR

Macromolecule #38: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 698.854 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #39: uL13m

• Macromolecule: Name: uL13m / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 24.341309 KDa

Sequence

String:

MQRSTRIVMA PFKSVLRRHK HHPEGLPLYN DMSKQWLCRE GERWWLLDAR GEQLPRVAAV AAQYITGQHR PDFTPGMITG DHVVIVNIK DVVMVGDQWI RVPITWQTAY PGGKYRIRLT DMYERDPCML MWWFLKDEVN RHFVRKLKTR IAPLEKAWLY E DSIHPHAD KNPRPLCWTD PEVKGWKYRD PQFIRRWKPN EFMH

Macromolecule #40: mL80

• Macromolecule: Name: mL80 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 34.883273 KDa

Sequence

String:

MCCLYTSDVF FWSSLVVSPL PRIVRCVSAP QIRSIPCGSG DFSVMKRSLI ARWQSGVHTP HGVVYRGAKM KNWPEQRIPE NFKFTEEQR FRTKAIPRDV GTIPRNFVLG VLYRHQPCEV GGLWEHCTND PEIVLDSKRH LREVLKQARE EGFVTFERDA I SNEWLCFL TRERYEEVQR IVTAKSEAVD THSGLRGAAA TETSTYAEKF REMNVEAKEA HARRLEEEVA NTTRYLRRFQ QR EIDYLPY TDLNGKVNFM WWYETRDVQQ RADEVLTDSS SSKALAGEEE HKAGSQLEAT TASTS

Macromolecule #41: mt-LAF14

• Macromolecule: Name: mt-LAF14 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 82.108977 KDa

Sequence

String:

MRRCGNDRAV ALLRATTEAM RLVKLKLSPD RTRNEEIQDR QNAFVWSDEH IFRPHQHFTH DPCSWSRSLE QSMKKQRKLS MVERLRSLE QRQLEEKQSA SATAGGSSKC ANHMDGEKAE GPRFYGAVGD SEDLKEYVAN EDYFYTMQQE EKPNDPPLQE L VDEVQSLH VLLSSPRYED TPLATVERLQ CAYSEALRCV FDRVRNASVG KTMSCNALLF SWSLLLQGVP ALLESLAEKR TE ECLVRAL STVHEALNIV LQEFNRITHS KERVELLPLE GWIESLDVVT HPLTNKDYTS LKGNIRLPES SFKPQCKLDS ATV EFVHSR AIQAAAIRMI ENDQSDVETE PLDPYHLYIL LRCMVRLAEK GVNDSHIHRA ALLTGMVGER IFSSLERTVA PPRR YSLRH ALLGKQLRDA SKPHAIPLDV CAPPGGVKKP PTAADDVLLL TRACTLLMKV ATNVLPQTKF KVLETVDTVL KTLSY APNY DLSTADTVIF SNMVLEELHH VDEASATDRH LRVLLLLSRL RLSMCADRSA LSHLFSCLCN LLPPHSIQQD KLREWK RLR GLVMRHLLYS VRGEEVEQHY TRVLKSSETW VEHLAFGQYS GGLPLSLWLE ACHIYLTAGR KLTVSCAEAL ITLRGRC KD GGVLRSSNSA GVGPLDFVSV TLLAQLLEVV SHGCCSADDL VASPVAWDKV RQTIQGAIGE DENTIQLLRA GRLCVADR Q ATGSLVTTYP

Macromolecule #42: uL14m

• Macromolecule: Name: uL14m / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 25.133586 KDa

Sequence

String:

MYLIICYIYI YIY(UNK)(UNK)IFIRR CLLQPRHLMF RFFSLRLAPA PSADAAAKLP AGKPANKSWF RHNLIIRRKA SY RSRWGTG AEGYGTGVPF SDQVKLHCVD NTNCKHVRLI SKATAERFAH CRVFPAVAHR VSVQRFKSGR GEVSRHRVKP GNI YWVCLL SRRQTNTRMS GLRTNFDRNT CILMNDQRVP LGTRVMYCAG RHVNHKYHLK AVVLANFFV

Macromolecule #43: mL81

• Macromolecule: Name: mL81 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 29.284467 KDa

Sequence

String:

MKRLFPSAGV SVVLTSSSIV MSCPCNHIFT SRRAYYWPYN EDFVPEGAET SRFQSSGSPG TRRRVLQEYA LSPLFGARVP CCVVGTLRT AKEIIVLKRD IQSLLCELME LPQGSVTLGP LQQLREMMLY RHGTPLSPRL GDERQLNMDA YARRPIAART M MDVFLAED MSLDEIVNFG RLSLGKLQIA LNNLRKENAE KSSADCEDGS AVEPAQLLVD RMGISYCEIP SLDESDYVFA EV GGVAVTD EEAERVAQRW AERCE

Macromolecule #44: mt-LAF15a

• Macromolecule: Name: mt-LAF15a / type: protein_or_peptide / ID: 44 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 34.621383 KDa

Sequence

String:

MFTVSCNVAF LCHPAVHHSL LLLRALRQRH TLAIERMGAN VTNIGGTVSL SQCGNHISIV PPNLHGSKCV TSGGSIGTVG ESPLCVAEH GLQRVHDPQH ILYLFSSASP VRQSALDGQI QSYLNAVVVS NQVLRAADDV LIALSIGEME AVRQTHGNLI D CVAALDAS LQQTTENEEG GGGNGATQEV DCLSTWPLFT TIQFLVEEGG LPLGPFPRMS RAYYRLKEST PVVAHSQLVW RT FELSRGP EGPTGELPAW PHRGFLRDIQ RQIAEYTTDP PERIMAGVTG EKGPLRARVS GARLGLQRTP ARIPWTMQGL HR

Macromolecule #45: uL15m

• Macromolecule: Name: uL15m / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 43.780016 KDa

Sequence

String:

MLSVSPPLRR YRLFHPRREA IPMHMCPAKT IFPLINSNNL LVKTRNSWED FTGRKEFDED HPLPVVGSRL NGRTTQHKWN HWDQYLNPQ ITQSIKDLTP TPEYVGMRCG HNMIKMGWMK IGGSWKYSRG YNDRRRVFAR GQWQERKMTP RFMLAPRVSA G GPRNRYEG KLVFSPLRLS KLLWAIDTGR INPNEVITLY HLRQANVVGE REIVWPGFVL ISNGVRRVPY PIHIELQNAS AE SIRLIEE AGGSFTCVYM THEGLYQELH PEEYPIFMDQ ELPERRGLES LATNPSKRGW LTRWYEDSSK YAHPAAGRRY SHY LKPPTE RDFPATVEEY EMVKHHQKWH LNQPGTGTLL PWHSYNTADL VKRAAGRL

Macromolecule #46: mL83

• Macromolecule: Name: mL83 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 25.579004 KDa

Sequence

String:

MLHRTRLAQL INGRILQYFV PYERDEVNPV VFMDVTVEGD ALGRVSVELF HDIVPKTTEN FRSLCTGERG YSQCPLFYKG IPFHRVIPG FIVQGGDILL KDGRGNVSVF GFPFPDESFE GKAGKHLRGT VAMAHSAPNQ NGSQFFFNLA RNDHLDGKFV V CGQVIDGW EVVDRVAVLS GSSCGTPVSR AWITECGQSS GDKLEDTERA LSGERALHTM PGKEVLDILS PRY

Macromolecule #47: mt-EngB

• Macromolecule: Name: mt-EngB / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 71.472609 KDa

Sequence

String:

MCICTRLLCC SGIGQARRQL TPASSCQKPW GNDPPHLMSP QDELVAKLSD EVFSRGNMHL KNVDVVSALP EGLHSFPEVC FIGKPNVGK SSIISCLLRN PRLGRAGRVR GTTRLLQFFN VGDALLLVDT PGYGGWKGRH LPQSVAERAS AFAILFRYLA L RSKGPLKR VYWVMEATKP VQPRDEEIFV FLRNEQIPFS IIISKLDYFG GDGAALRRQV ESIYNFLGTE DVPVLGVRAD SS RPERCIN MTALQHDITH YCTTDLVRVE DLSYSGLKEL SYAPPTFDEV RAVEERYPVE SFIVPQDDNL SLQHFVSLHQ EAK SRHLAA SPMAMRLSTK EKLGANLIGD DGGSKGIDID EGTIVAHSPL LARLTIAAEG TI(UNK)DSSLLEG VPLGVVAVSP TKMSQCFHE HPSIREGLLR TSRVGHTDLQ REEAVNRVQP VSPCPSPTIK HE(UNK)SEHLSCK PTGSLGGDAP GASPFSS SR FGAHMETIRT INGVCIPKSM VPPSVVQLAA GQAGSFAAFA QHSGANAYEE FLTGDATGSG TFLEATGSED TLENKGRS L RRPREKSMRR CALDKVLKRY VACGRKQRSL HMQAEGYMCP WLAGAGQQAR SAVFGVTRSR AHAGGMEVLK GLKRTGFGG QSYSARTMKN RGRSTKKTGF WAA

Macromolecule #48: bL17m

• Macromolecule: Name: bL17m / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 35.117824 KDa

Sequence

String:

MLRITLLVVG RAPPVIGCTR KARYAGIDDN PTITYKPWDT TEPLMADYGW TRGKLPKFRA RSPFHRQQIA RRMVTEMIRK DYCIVGGAR APALRILADH VVELAKAGDT DSRQQLAYFL HDPLMVDKAF DEYPRRFKDM NAKYAMMTRL KSRRRTDAVA M YFVEYKNR DMSDNHKGED YSAGPERFFL PPRIVETEKG IQRPPHMQMA FDRWASKFKT EEFHHWWRLR HAKLRYWGVR NV PHPSDVD PLWTEKEEEE WHNEMLANTG EYEDLDLDDE SYTAAGEGGE PSPAGDGPQP QRVG

Macromolecule #49: mL84

• Macromolecule: Name: mL84 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 24.773643 KDa

Sequence

String:

MLRFTRLFRE MAPELQLEYM PVLFTRTILG PQGGFAGEER LVKLEVARKY MEAGHAVTPT EELRRGLWCY NPDTDKYDCF IERNEEFLD FAARKRQWLD VYWRVNTGYL LFGRQSWGQG FLINCPLRKR DVAQKLWEQY KVRIDPRLIE FREKDRRTGI Q ELGHNWCW LYLPGAEELG INREVYDNKR VKVRIHVRKM NSMFALY

Macromolecule #50: mL85

• Macromolecule: Name: mL85 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 22.657951 KDa

Sequence

String:

MRRLGVFCRG QRNSPLLTRG IATGGRVTNE DRRWWLVHLE CAPDITPGTF IAWLDCCGTH TCKKLIERNI WTIEQVAALD SDQVDELKY REGCLKMDVV WEHARTIITP LRQREVTGGV ESELQGRIME LRKKRELERR REEILKERAN VSEQREETLR K LREAIAAK KAAM(UNK)QKKQA ASEAYGGSSD GGARKEGAEE

Macromolecule #51: mt-LAF18

• Macromolecule: Name: mt-LAF18 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 58.830738 KDa

Sequence

String:

MSVPSAIRID TRVAYRRLIK VADQVTRDCP LYQTEGLRQY VGRRFLERAE RNRQQYRHLL HDGSGDARGG RRKFQKQRVL TAQYGKFIA KELQQVHGLA ELLLRAPGNT ALTSMLQVLS AGVGNVHYQQ TVERNYVRYC QFEEKKLDRD EVEEEATSDR Q NRIMQHAL IPYGERLLIL HRLQETGGGR DAVAEPGRLT SWQVTEAIVG TRSGVTAHHM SHGKENVVVE VDETFNKQIV YV RCETYDW SRDVERVEIA EAEELRGTVF HAEYLSVALR LCDALMVETP LHAYRSTVLV GHGVGGAVAF CLALLLHARG FDV KNCVTL GAPKCIQKSL ARYVHAVNPI RVVLEGDPLI ELPVTGAEGD PFVHCGEILL MDNRARADLG SEADMKTREA GRQS EGGRN ESTEDLFTAE SLSDSLDPSE EPAMGNEEGC VATGDNNDEG DVGGEDLLEQ LRLAAKRYSE GFLVNHYVEH LTDTT VRLT YAEGDEVWDE GDYAQMKRED QLGHSFSGSQ KWQDELRSPV

Macromolecule #52: bL19m

• Macromolecule: Name: bL19m / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 16.931455 KDa

Sequence

String:

MGYTRERTNR HFFVARANAF FSRLPIARVQ RSLAMEAVRE GRMRPWKYTK EQILGAPVTC NFEYNPRPVR LIGTVMDAHT EETSIKGGL KVYARNEETN MMLWIPPGNP KLRHEVTSTK GSFQHYLDER DKWDEAWITG RARMK

Macromolecule #53: mL86

• Macromolecule: Name: mL86 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 22.376191 KDa

Sequence

String:

MRRCIPARGG FTMKYKKGTG LWDEDHVNDY KTNRYLSARA TMRWYQEMER HQTRNSLNAR RATQSHNNNR GLHHTGRGAF ERELERRGV QVEKYPLTTT TGTMRVAELV ILRRMELEKR AEEALAEQRG ELQKKNPTPS EWYDESKGPL NPNFLRSMRS H YEVDIANL PDTPLIRGQR EFFIGEERGN GAA

Macromolecule #54: mt-LAF19

• Macromolecule: Name: mt-LAF19 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 12.076086 KDa

Sequence

String:

MAFRRLVKRH KITNNQMLLM RRREPYKPTM KDRQEIADRA KLEEFERKNA DGLMFVPEKA LPPWQKSLAH NAKALGSRIN FRGFRVRVA DGQDEPGFPT PFR

Macromolecule #55: bL20m

• Macromolecule: Name: bL20m / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 24.393943 KDa

Sequence

String:

MLRRTVCVQH YRAKLELDRI RSMLRGRARL ERKVGLKRLF FLMRTQTRYR VEQQAHWERA IVRKNVDSAA REHGTGWQHL RNELGRQNV MLLPRSQQLL AQYEPLAFRA VVELCASRIP PPPPPVVASV PEESYTLWPP ASHDNSECAS TDGSDAPHGQ Q QSLSHPAA RVELRCGVER VLRRGPSGLG NNVNELIDAW KEFDVSPLRK GEVNK

Macromolecule #56: mL87

• Macromolecule: Name: mL87 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 22.337521 KDa

Sequence

String:

MLNPTFSLYR KTLQSYPVPP KIRHYDRRWS GSRTNPYNRQ YWRVIMNENY SRPSFWVSDF RHRYLMRTGT DYQGQVPSSP QPGLYQGFS DVHKLLANHP KPQRESRHLP VLPMTPRIVF EHANEKRIDT AKRMRRDRRR IEELKTLEFW GWYMKLQRVR G RWCREQGV SSRGVYGPAV DAAELWG

Macromolecule #57: mt-LAF20

• Macromolecule: Name: mt-LAF20 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 6.939401 KDa

Sequence

String:

MFTPSLALWS QFLKRTFVGG MGYNVKRPYR IEIRMEHDKK RRMRRRNIGC RRMMKS

Macromolecule #58: bL21m

• Macromolecule: Name: bL21m / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 20.895268 KDa

Sequence

String:

MPSLANGLRC PDALITRTLN PLLNGPKFAV IYCGNHQYKV AVGDVIAVQR LRVEIGSHIA LKKVLMVGGP RFTAIGRPLL EGVRVTAQV EEQKRMRNVV SLFATPGRRH VRWVDAPHAA TIIRICEIFY SPNVVGELDK YKGELLETFT PGQHTNPVYP V DDGYDVFR KKDKEAMENA TTFLDLLGGV

Macromolecule #59: mL88

• Macromolecule: Name: mL88 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 22.25559 KDa

Sequence

String:

MLQINAFKLV RATPFLLKRT GKPADTPDYK QVYLPYDAAP TERELERERR RFKQAYHGRM EHRKLVEVKE VPLNVYTYGK EGMSLPIAI FKDQKDPVIG PEWTYPGIYE NKIAAQHWYT EELFDKESKE AFESPWQQQI LDNQVKRRMA KVMFRMRQVN M KAVDLFQK ERGSSRRSGG AGEKGKDGGG KK

Macromolecule #60: uL22m

• Macromolecule: Name: uL22m / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 32.083285 KDa

Sequence

String:

MPRPAPRFAM GHMLPHRSNV GSQFLHTQRH GSRSTWYKKH YFSLRPFAIQ RHHGTTPRIL LDRSLWKSLW ITKLQLPDIN RWERVVNSR RVTEDRYAFV EEEGVMHKVN WGLYCERLET ELTVTQERLP QHTLLMKAVP SSWKKLDIDI SVIRGLSLRE A MAQCKLSL RKGHQIVFRA LEMAQQGAEA KGLDKEHLRV AHISCYPGPT DKQIDIRSKG YYAWKTKKSS HLVLTLAEDP EM VLPDRTC LPYSSLMSMK RAGLSAQPTV IDVPAITADG I

Macromolecule #61: mL89

• Macromolecule: Name: mL89 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 22.213406 KDa

Sequence

String:

MSGAFGCGSY RSVVAGTQNV PRRMTFYPSA YELIQLHKAH REVIRHFYVR DKIFDNKFPG NALANGLFKF VPNRRENYHM RELMESIRR RSIWMHRIKQ QREINAKVVE NMEVKYGKKA AASMLCFTTP DSNAYFAPHR YQDVANSWPN YWQHPSVNHV V PKPRWRRH RELGGITRVE DPFAVQASDY

Macromolecule #62: uL23m

• Macromolecule: Name: uL23m / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 28.847963 KDa

Sequence

String:

MVVSLRRFHS YANSSRGGAT TCLITGGGKG HFALTVSTRG RFYRPLVDDG INLWRRRMGR IHKGWRTWEY QHTRPDPRPF PDPPVNDYF GRSRIWNPIA CKLGYVRKKA DEWGWPNKPP PPTGLRYSQE FFPFFFERYF PDAEVRLLLD SVLNNETKRP V FQIPCSMS KVELVNYLKN IYGIDNVVRV EVRNRRGRRY KNEVGEIKMM DDYKVAVVEL DTPVSVELKQ IKGTEDTSDN RP QERITQ

Macromolecule #63: mL90

• Macromolecule: Name: mL90 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 22.123414 KDa

Sequence

String:

MLRSRIPCVV VTLVQCRAMH GKPTQSHKVR TQHSRRWWTQ SKARHLTAMP HDECKSRPHF PAYNEDVDRP MVVPPDAICF NCDKPIDAG DVNSYVWIPA GNATVPTPQG YFFHFGCFKC NRCKYRLGHN KFYSKDGKAW CIPCALGRDV RVPTRRWHTS Y VNTHRTGS RLTGHFFPRH SHQMEFLFDP NS

Macromolecule #64: UNK

• Macromolecule: Name: UNK / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 15.336877 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)

Macromolecule #65: uL24m

• Macromolecule: Name: uL24m / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 43.999047 KDa

Sequence

String:

MYARFRTRSK FYFRPARPAL AYNVDPNVMR RPKVKRGLLK GTYSDETVDL RDRERLELLE SMRHPRERDF YQDHTYHNQW LRRDLEKHQ KQQLAARYKY FAPDFEISPW IWYPGDIVEV VSGEGIGQRG TIIAVIKYKN EIVVQNINVQ DVVIPASESR P EQIVQREH PISVTRVRHV DPSTNEICNI EMVKVRNKET GEMEEKRMSL ESGILMSIPP VNDELEVGDP LKDTPIQDAD EA TYDREAE QAVLVDKRLE AMEEHFVQSL KQSYEFHEPL RRKNAEDMRQ FQTDVIDMAC AMLGERLLDT VNASDTSSFP AEW QEAIAM HVEEIEAEME EVAAKEMAEA KEGGAAENEQ HDDDLDDDED ELDTSEESVK HV

Macromolecule #66: mL92

• Macromolecule: Name: mL92 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 20.542291 KDa

Sequence

String:

MRISERAFMD IAIGTKAPRR IVFKLFPRKC PSAVKNFIEL CSGNVSTDTY ESGNRDKLIS ESALPQLTYK NSTFHRVEKG YLIQGGDIV TGRGTEQLSI YGGTFSAPEE VRASVFDKPG LVGTASSSPN AHGSQFFILT AKEANHLNGT CICFGQVADG L DVVQEIEQ VPIDPSGFPS LKVSIVDCGV LE

Macromolecule #67: mL93

• Macromolecule: Name: mL93 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 18.929691 KDa

Sequence

String:

MFRVTGLQLK NPVVFKQGQG MFSHQLKRLL QKKSIHRYNW DPLPMYDPRK LVHASRHMDV ETWREVPDPH WDERSYLVPD QMFYNIPVP PEYKDAYWWR ELQARRVQCP VEWVSHRMYN KGDRQRYDFQ DLAFRKKFEF SYEEVVKNAK DMRS

Macromolecule #68: mL94

• Macromolecule: Name: mL94 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 17.830959 KDa

Sequence

String:

MAQWVPKTAW KVSNLNKRYG PGYITSGFSS ENTLGYTHMV HDSHKLREKL SSRKGIFVID VRDPGERKLN PVPRSVALHH HDLLSGASC PILPQQKSAA ELFVLASNES RGLNSAAALR RWGYDSVVQV DYNTLVEAGY VSRCDYATAG TKDSAEQAVS G ED

Macromolecule #69: mL95

• Macromolecule: Name: mL95 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 17.803377 KDa

Sequence

String:

MFRPTTAIAD SFKEHYHRVH LPRRLALQRY ARQQSLRNAA KGNVKAEEVP YKYNRWWVNE EHEFVHQYAF VEDPEVTKAK RETLPPVTR ENIWKEPQQT FFLPFAPYVR VVDYPKDPDA KFLKPVNIPR WKDYMQRTKP VIPRTWY

Macromolecule #70: mL41

• Macromolecule: Name: mL41 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 22.610928 KDa

Sequence

String:

MLRCSCARRR GVYHNAPSVY PFVKPFHDTP YDQDRGRHDS VGQRYRKNSW PEWMDNGADG TGYGIGLHRT HPLSRLRGNL KRSPSHVPR VLGMMIQGVW HKSGVKLYFR GGKPPNPSVH PYLTGEPCPL YGWKVTDESV IRQFNMPSID GTNFRYKPYV A LQERKIMG VQVPSDSVSL ASGRTTESKP LAKRLFFWR

Macromolecule #71: mL42

• Macromolecule: Name: mL42 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 21.602639 KDa

Sequence

String:

MLRLCRVSLR VQSHQKKRAQ HPNAGTRFGR VYNRGFIRYG FGGFGMSVYT PKKDRRFRVQ PLPSLHANSL ADDTPLVTTT RTLSPNFRA FALQDGGVFF THPSHEQVMR VGQNILAEET KATGMTSMDT YVNSRIQSII AENTVENVAL SHWRRRHMWN L VRTHGKLQ RHWGVSDATK GARSNIYGRP S

Macromolecule #72: mL98

• Macromolecule: Name: mL98 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 12.968644 KDa

Sequence

String:

MVLRGVRLRS VAVSCYGSSL TAATRCLSVR TEDFFSKEAI SHARRVSWAP HTTEKKQGAF AKLARSNFGD PLPSSFAQEP YFEEEIEAH RKHHRPDVYI YKYNVSPTHF SLRE

Macromolecule #73: mL43

• Macromolecule: Name: mL43 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 31.300406 KDa

Sequence

String:

MSRNGELCLK KVIISYCPSN GAPNTRQFLA THLPHFHAKY PSVSIDLRPR LWPEMAITGV YRDGSERSYN TKNLSPMGIF LRLNNLVST ANDYDQPFCA SHLHFQRRSV QGTWNPWLWN YETERRRTEA PQWRRKLSEK EWDYYVGQYS AQMKQEEDEI Q RRVADRTC VQEQSTREVQ ERWKRHVVPR MQTDLEFNLS HFKRQHARGQ LQQRPVTMGE YRLFSVPDPR ELGQDAVDTM RR RESHNME VWWRKRKEQL KPP

Macromolecule #74: mL99

• Macromolecule: Name: mL99 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 12.334969 KDa

Sequence

String:

MYQRTRFLWS SWRDYPLGSR DRRGRFNMDE AAAALQLNPA YAAALYRPLN YTFHIRGQLY PAQKGRPSRP GSLAASQGRM FPLYQRNDR LDKELFRLNS RGLTTE

Macromolecule #75: mL100

• Macromolecule: Name: mL100 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 10.600362 KDa

Sequence

String:

MALFSCFRCG YMYEFAVSNS YCRKLTLRND HCPRCDQLTL FRFMSVSGMV GNMPFKPIGV PGPSYATLWW RKTREGKEAS APLDAVCKS DRW

Macromolecule #76: mL101

• Macromolecule: Name: mL101 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 28.455811 KDa

Sequence

String:

MLVRTWMLLN RPKGPQGLRP GKEYRLTVPY RSEVTMLRLA NHKAINSNIR ELFKKPLVMN NIKAIPRDLG EIPRDYVLRL LFFHQPIRL VDLWTICKEH DDVPLDSAKH LRLVLKIAKL QRWVYAEKNQ TNNLYYYYVH QSRMQEVQQM VRASEVRKKE Q ESVREIEA EKLRMEEQER RKVALDENIV ALQNALVSNI AQIQEFDPGF ARSKIYVTES GAVNVGWGLN DGGSAACSSD LD GQQVA

Macromolecule #77: mL49

• Macromolecule: Name: mL49 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 24.501486 KDa

Sequence

String:

MLNRSRLRRV WLGGTAANTS LGRDVMPSTG KYINIVSPTD VAANISAGAM PPEANVAAAL PVPRIQYSKW NRALRSDVYD ELLKLPLRY KLHDFAKLCP PQRCQEEPEA VGVVGRDSAV GYLPPLGPAD PLDTIPFFVH RSSNGVLPGK VFSMHPRNLM P AFYLRIQQ VEGDMFRFEE ELLKIFPTKK IFVRSHSIYV YNVALDGRMV LHHWLLGLGF

Macromolecule #78: mL52

• Macromolecule: Name: mL52 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 167.692812 KDa

Sequence

String:

MHIWRTAASN WVKRHARSNA AQWRRPEPAS SSAAAKTIAR LLAVGGHGCW SEATALYAEA LATKSVQRYG VVTPAHRHDC IALILGAMS SSVDASGSDI NRGEASLTPH YGMLAERLSL DIIVPHSDVG QEEKEAASVA CIAVFRALLT SGRTHAAQRF A QQLLRSRN SREHGLWALR TIMLAVTARE EGMYRQLFLH GGLLKDLEHL TGLDAFTVVM SSCDGHEKNI GPCGGRTEGN LG RDEVHRV AARWCVDVAL HMHTCPWRFC LTNEKEPRRE TAFNSEVTSY CCAKELPMPA FAVTMLPFEV LRSNAVGVLR CCT AAGNGS HGNEVVTFDG TASNVSGGRS GDSTSCSTTA PSLGLPALKR LQDVVSAAIE VPLSTHEPVE CLYLLLQISR DSTA PDPRR VMPSTMNILW WRRHLQSCVL ESVKSAVRWK SVCDFVGLSR LVTERVPHCF PLLVAHAAES PDALRTLFNE VSRQE GAAA (UNK)PVDDIPRSC VMGLLQMCGD ERSAAVIDWV VEHC(UNK)DHESI AQFVLDSLGQ HQELASVVVR RLFQRSEQ S (UNK)DVINALSRL LEKGTCRTLL ADVCSLVPQA QQWTVALRFV SGMSAPEVGH VHTAFMAFVG NCDEQFAINM ALLWS DETF TWLKADVNPP PRRVYMDDRW SDALRLVALG AERLTQRPEL LGKYIRYFSK RVAVNSRLYM ELEALLACSK VGGGSA RNC GRSSNWNPIA STVTGGPPLE TSLVGVRTEV LPKGRQWMEA CAHASEVGVT RGVLEILAKR GRWEEATILL ARAEAKR RV EWAPLVIRAA RLSSQWRAAL SVAEKIAANG MKLHYSVVAE LLACCFSADV PLEVVQLWLH RRSVGESTSS HVLFGLGP G SSLRTEQRDA NDKHNVFGDA SRWLGSAQAE LFFVLLESSS SRSSDHQWRY ALQLLKEHVL LSGGVPSARV FRSTYSILH RAERWRESLQ LLGLQRSVCG SPTVKCVHLV LSTLPSTAWQ YALGALQCIP PGDSGSIHRV LPLLLPVSWE SALGLMIDHR VMTNTAMEC VVGCEDVPLA LRLQAWRRLL PSLPVSMKHR AAPIYLRLAA GVADGDVGLE GTNGLDAMCV IERSIRGLRG D YINYASFV YHRALLHRHW CNDSLHPSGG VAAFRALSGI AEVDQQCEVS AAALEQLSNI VERLEQVCGT VSASTATHGS QH LERTVPV VNSGEILQGS APTNARRDPE THCFSFVSSY WLSLFYLFRY CRCVISSSLF PFFKKDQGSG LPIFDVMRLH TVR APIITR AAMRGYSEAR SNYDGTSLPA WPAPGKKPTY PAALSELRLP QPRMRKTRTE WMYYHGHGGC PGKYGPSREI ADFE YADGT PASISGRRFA FKHHQDHLLV QLIRAAATVE RYDASGLLPR IPGTAEQRNW DPAIPLFLDD VDEQGRPAPL RTAGD APGT MVSHVCSRVV DERMGTPTHT PNELANRHEG ETLEANTMFA TNDPSAFVSD TVKLRDDKRP YWSRRRWALT DKFLVP KSP KPKNTIKDE

Macromolecule #79: mL53

• Macromolecule: Name: mL53 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 35.656383 KDa

Sequence

String:

MLNPPKHYSV ESLRTVGLLP AQLALSRKPR LRPHVGNLKG LVYPLPYYAM WRGNHNKYTY NKSTVCLWGE GDTRSMYHQH YAHAKCPTD YGRGGREFEY LTVKRGKMLQ KPLPRVQYVA EGSKPVWLFK SWHTPLSSPS MWEREVQYAE HTPEHIGAKR P LAVVAPRT MHRYLFLMHM EKVTITVSPL LFGYGHTIQK AVLDFYRRAI SARSPFPKDK VFLFYAIDHI TPRIEVTWLD GT SYVPPVL EGASSQDLIQ MVMEEAWLAA DRMAAEGRVL NPLAIDDYKW DQLVVFKKVR DKEASKGGGR KK

Macromolecule #80: mL63

• Macromolecule: Name: mL63 / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 18.213924 KDa

Sequence

String:

MLRHCTAHRR YRTAWRELLH PLPVRARKME WLKRDAVEEN EEILRRPYYT IKSYALPPAV GRQESIHNSN NIRGGMHSSH SLDLIMRQP RRVKTPEQLR ALRDRLRFIG VTGPMPQATS VSTKSYTDTY GSRLRPRYPE SWDTVPPHQP SRELL

Macromolecule #81: mL64

• Macromolecule: Name: mL64 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 28.545977 KDa

Sequence

String:

MLRGTRGFLA VSPGVGIAPE TTPVKYTPMM LNIQNMMWWN GKRNLYRATY REKTWYEISR TGAFTKGRRP VMRQKYSREA LQAALAMVP PGFEVADVPR PPQRILAQSE GIVGRWYSNY WTLHSMRYQC LLAGVEWPLG ERQRPRTNYD EPFFFADFEE S KAIRDYRS RWINVNRSLV GMTKRMKEAE EEARYMQFRK LQDTFWSNRK VLVNRVKSMY NQGARTSAKD MPIKTINIKA FL SE

Macromolecule #6: 12S ribosomal RNA

• Macromolecule: Name: 12S ribosomal RNA / type: rna / ID: 6 / Number of copies: 1
• Source (natural): Organism: Trypanosoma brucei brucei (eukaryote)
• Molecular weight: Theoretical: 373.911656 KDa

Sequence

String:

AUUUUACCAA UUAAGAAGAA UAUUAUAAUA AUGGGUGUCU UAUAUUUUAA AUAAAUAUUU AAAUUCCGUG UAGUAAAUUU AUUAUUUGU AUUAUUUAUA UAAUAGGUGU AUUAUAUUUA AAUUUUAAAU UUGUUGUUUU AUAUUUAGAU ACAUAUUUAU A GAUUAAUA UAUUUAAAUA AUAUUUUAAA AUUUAUUGAA CUGUAAUUAU UAGUUUAAUA UUUUUAGUUU GAUGUUGAAA UA UUUAAUU AAAGAUGUUA CAGUUGUUCU AUAUGUACCA AAUAAAUAUA GUAAGAUUAU UUUAGUUGAA UUAAUAAAUA AAU AUUUAU UUUUCUUUGU AAAUAUUAUG AACAAUUUAA AAAUUAAUCU GUUUAACUAA AAUGUUAUAU AUAAUAAUCU AAGU UAAUU UGAAUAUUAA AAGUACAAGU AUAAUUUGUA AUUCUAAAGU AUUUUAAUGG UAUAUUUUUA GUAGGUAAAU GAAAA GUAU AAAUGGAUAU AACUUAAUAU UUAAUAUUUG UUUAAUGAAA AGUAUUUUAU UAUUAUAUUG UAUAGUAUUA UUAUAG UGU AUAGUUUUUU AAAAAUAUAA AAAUAUUGUU AAUAAAAUUA UCGUAUUUUA AGUGCGUUUA UUAAAUGCGU UUGUCUA AG AUAAUUAUUU AAGAUUAUUC UUGUAAAUAU AUUUAAAUAU UAAUAAUUCU UAAAAUAAAA AAAUAUCCUC AAUUGCAA U AUUAUUGUAG CAUAGUAAUU UGUUAACUAA AUAUUAAAGU GUUCCAUAGA AAAUUUUUAA AUUACAACAA AUAAAAUAA AGUAUGAAUU AAUAUCAAAA UUUUAAUAAA AAUUAAAAAA UUAAAAUAGG GCAAGUCCUA CUCUCCUUUA CAAAGAGAAC AUUAUGAUA UGUAAUUGUA UGUUUGAUUG GGGCAAUACU AUAUUUAUUU AUAUAGCAUA AGAACUAUAU UCUUUGAAAU U AUAAAAGG UUCGAGCAGG UUAACAAGCA UUAAAAAUAA AUGUGUUUCA UCGUCUACUU AUUACCAUGA UUG(N)(N)(N) (N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)AAUUCGUU AGUUGGGUUA AAAUCGUUGU AAAGCAG AU UUGUUUAUAU AUUUAAUUUU UAUAAUUAAU AAUAAUUAAU AUAAGUACGC AAGGAUUGAU UAUUGAAAAA AGAAAGAA G AAUAUAAUUU A

Macromolecule #82: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 82 / Number of copies: 5 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #83: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 83 / Number of copies: 33 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #84: SPERMIDINE

• Macromolecule: Name: SPERMIDINE / type: ligand / ID: 84 / Number of copies: 1 / Formula: SPD
• Molecular weight: Theoretical: 145.246 Da

Chemical component information

ChemComp-SPD:
SPERMIDINE

Macromolecule #85: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 85 / Number of copies: 3 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #86: SODIUM ION

• Macromolecule: Name: SODIUM ION / type: ligand / ID: 86 / Number of copies: 2
• Molecular weight: Theoretical: 22.99 Da

Macromolecule #87: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 87 / Number of copies: 1 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Macromolecule #88: S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYL...

• Macromolecule: Name: S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE; type: ligand / ID: 88 / Number of copies: 2 / Formula: PM8
• Molecular weight: Theoretical: 496.598 Da

Chemical component information

ChemComp-PM8:
S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE

Macromolecule #89: NICOTINAMIDE-ADENINE-DINUCLEOTIDE

• Macromolecule: Name: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / type: ligand / ID: 89 / Number of copies: 1 / Formula: NAD
• Molecular weight: Theoretical: 663.425 Da

Chemical component information

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

Macromolecule #90: water

• Macromolecule: Name: water / type: ligand / ID: 90 / Number of copies: 10 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Grid: Model: Quantifoil R2/2 / Support film - Material: CARBON / Support film - topology: CONTINUOUS
• Vitrification: Cryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV; Details: 3.5 uL of the sample was blotted for 7-9 sec before plunging.

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 75.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 98508
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD

Atomic model buiding 1

• Details: Initial models of ribosomal proteins of the T. brucei mitochondrial ribosomal large subunit were taken from PDB 6HIX. Assembly factors of the mitoribosomal large subunit were built de novo.

Output model

PDB-6yxy:
State B of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate