6YLS

mEos4b - Directionality of Optical Properties of Fluorescent Proteins

Summary for 6YLS

• Entry DOI: 10.2210/pdb6yls/pdb
• Related: 6YAD 6YLM 6YLN 6YLO 6YLP 6YLQ
• Descriptor: Green to red photoconvertible GFP-like protein EosFP, CHLORIDE ION, SODIUM ION, ... (4 entities in total)
• Functional Keywords: meos4b, fluorescent protein
• Biological source: Lobophyllia hemprichii (Lobed brain coral)
• Total number of polymer chains: 1
• Total formula weight: 26947.84

Authors

Myskova, J.,Rybakova, O.,Brynda, J.,Lazar, J. (deposition date: 2020-04-07, release date: 2020-12-16, Last modification date: 2024-11-06)

Primary citation

Myskova, J.,Rybakova, O.,Brynda, J.,Khoroshyy, P.,Bondar, A.,Lazar, J.
Directionality of light absorption and emission in representative fluorescent proteins.
Proc.Natl.Acad.Sci.USA, 117:32395-32401, 2020

PubMed Abstract: Fluorescent molecules are like antennas: The rate at which they absorb light depends on their orientation with respect to the incoming light wave, and the apparent intensity of their emission depends on their orientation with respect to the observer. However, the directions along which the most important fluorescent molecules in biology, fluorescent proteins (FPs), absorb and emit light are generally not known. Our optical and X-ray investigations of FP crystals have now allowed us to determine the molecular orientations of the excitation and emission transition dipole moments in the FPs mTurquoise2, eGFP, and mCherry, and the photoconvertible FP mEos4b. Our results will allow using FP directionality in studies of molecular and biological processes, but also in development of novel bioengineering and bioelectronics applications.

PubMed: 33273123
DOI: 10.1073/pnas.2017379117

Experimental method

X-RAY DIFFRACTION (1.55 Å)