6YLS

mEos4b - Directionality of Optical Properties of Fluorescent Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.541870
Spacegroup name	P 21 21 21
Unit cell lengths	38.510, 70.510, 90.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.440 - 1.550
R-factor	0.1866
Rwork	0.186
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s05
RMSD bond length	0.014
RMSD bond angle	1.765
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.440	35.440	1.590
High resolution limit [Å]	1.550	6.930	1.550
Rmerge	0.065	0.041	0.324
Rmeas	0.076	0.045	0.455
Total number of observations	151232
Number of reflections	61581	766	1747
<I/σ(I)>	9.15	33.75	0.98
Completeness [%]	89.0	98.6	33.9
Redundancy	2.456	5.666	1.226
CC(1/2)	0.997	0.999	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	PEG 6000, tri-sodium citrate