6YLS

mEos4b - Directionality of Optical Properties of Fluorescent Proteins

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Green to red photoconvertible GFP-like protein EosFP	polymer	235	26889.4	1	UniProt (Q5S6Z9) Pfam (PF01353) In PDB	Lobophyllia hemprichii (Lobed brain coral)
2	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
3	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4		water	water		18.0	198	Chemie (HOH)

Sequence modifications

A: 2 - 226 (UniProt: Q5S6Z9)

PDB	External Database	Details
Val 3	-	insertion
Arg 11	Lys 9	engineered mutation
Lys 13	Asn 11	engineered mutation
Tyr 36	Phe 34	engineered mutation
Thr 41	Ser 39	engineered mutation
5sq 64	His 62	chromophore
5sq 64	Tyr 63	chromophore
5sq 64	Gly 64	chromophore
Val 69	Ala 69	engineered mutation
Lys 70	Glu 70	engineered mutation
Asn 74	His 74	engineered mutation
Asn 102	Ile 102	engineered mutation
Tyr 121	His 121	engineered mutation
Thr 123	Val 123	engineered mutation
Glu 158	Thr 158	engineered mutation
Ala 189	Tyr 189	engineered mutation
Ala 195	Cys 195	engineered mutation
Gly 227	-	expression tag
Ser 228	-	expression tag
His 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	26889.4
Non-Polymers*	Number of molecules	2
	Total formula weight	58.4
All*	Total formula weight	26947.8

*Water molecules are not included.