6YJY

Crystal structure of human glutaminyl cyclase in complex with neurotensin 1-5

Summary for 6YJY

• Entry DOI: 10.2210/pdb6yjy/pdb
• Related: 6YI1
• Descriptor: Glutaminyl-peptide cyclotransferase, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, SULFATE ION, ... (9 entities in total)
• Functional Keywords: alpha-beta protein, metalloprotein, transferase, intermediate
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 2
• Total formula weight: 78407.40

Authors

Funk, L.M.,Sautner, V.,Tittmann, K. (deposition date: 2020-04-05, release date: 2020-07-01, Last modification date: 2024-01-24)

Primary citation

Kupski, O.,Funk, L.M.,Sautner, V.,Seifert, F.,Worbs, B.,Ramsbeck, D.,Meyer, F.,Diederichsen, U.,Buchholz, M.,Schilling, S.,Demuth, H.U.,Tittmann, K.
Hydrazides Are Potent Transition-State Analogues for Glutaminyl Cyclase Implicated in the Pathogenesis of Alzheimer's Disease.
Biochemistry, 59:2585-2591, 2020

PubMed Abstract: Amyloidogenic plaques are hallmarks of Alzheimer's disease (AD) and typically consist of high percentages of modified Aβ peptides bearing N-terminally cyclized glutamate residues. The human zinc(II) enzyme glutaminyl cyclase (QC) was shown in vivo to catalyze the cyclization of N-terminal glutamates of Aβ peptides in a pathophysiological side reaction establishing QC as a druggable target for therapeutic treatment of AD. Here, we report crystallographic snapshots of human QC catalysis acting on the neurohormone neurotensin that delineate the stereochemical course of catalysis and suggest that hydrazides could mimic the transition state of peptide cyclization and deamidation. This hypothesis is validated by a sparse-matrix inhibitor screening campaign that identifies hydrazides as the most potent metal-binding group compared to classic Zn binders. The structural basis of hydrazide inhibition is illuminated by X-ray structure analysis of human QC in complex with a hydrazide-bearing peptide inhibitor and reveals a pentacoordinated Zn complex. Our findings inform novel strategies in the design of potent and highly selective QC inhibitors by employing hydrazides as the metal-binding warhead.

PubMed: 32551535
DOI: 10.1021/acs.biochem.0c00337

Experimental method

X-RAY DIFFRACTION (1.67 Å)