6WC0

Crystal structure of AceCas9 bound with guide RNA and DNA with 5'-NNNTC-3' PAM

Summary for 6WC0

• Entry DOI: 10.2210/pdb6wc0/pdb
• Related: 6WBR
• Descriptor: CRISPR-associated endonuclease, Csn1 family, sgRNA (95-MER), DNA (30-MER), ... (4 entities in total)
• Functional Keywords: dna endonuclease, crispr-cas9, hnh, ruvc, rna binding protein, rna binding protein-rna-dna complex, rna binding protein/rna/dna
• Biological source: Acidothermus cellulolyticus (strain ATCC 43068 / 11B); More
• Total number of polymer chains: 4
• Total formula weight: 152255.02

Authors

Li, H.,Das, A. (deposition date: 2020-03-28, release date: 2020-11-18, Last modification date: 2023-10-18)

• Primary citation: Das, A.,Hand, T.H.,Smith, C.L.,Wickline, E.,Zawrotny, M.,Li, H.; The molecular basis for recognition of 5'-NNNCC-3' PAM and its methylation state by Acidothermus cellulolyticus Cas9.; Nat Commun, 11:6346-6346, 2020; PubMed: 33311465; DOI: 10.1038/s41467-020-20204-1

Experimental method

X-RAY DIFFRACTION (3.61 Å)