6WC0

Crystal structure of AceCas9 bound with guide RNA and DNA with 5'-NNNTC-3' PAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	81.635, 111.143, 177.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.220 - 3.610
R-factor	0.2439
Rwork	0.237
R-free	0.30360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wbr
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18rc4_3812)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	177.620	3.950
High resolution limit [Å]	3.610	3.610
Rmerge	0.198	3.629
Rpim	0.058	1.003
Number of reflections	252772	63055
<I/σ(I)>	8.3	1
Completeness [%]	99.9
Redundancy	13.1
CC(1/2)	0.997	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		303.15	0.04 M Citric acid, 0.06 M Bis-tris propane and 15-20% polyethylene glycol 3350