6WC0

Crystal structure of AceCas9 bound with guide RNA and DNA with 5'-NNNTC-3' PAM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR-associated endonuclease, Csn1 family	polymer	983	109589.1	1	UniProt (A0LWB3) Pfam (PF18470) Pfam (PF18541) Pfam (PF17893) Pfam (PF17894) Pfam (PF18525)	Acidothermus cellulolyticus (strain ATCC 43068 / 11B)
2	B (B)	sgRNA (95-MER)	polymer	94	30478.0	1		Acidothermus cellulolyticus 11B
3	C (C)	DNA (30-MER)	polymer	30	9127.9	1		synthetic construct
4	D (D)	DNA (5'-D(*AP*TP*AP*CP*TP*TP*GP*GP*CP*G)-3')	polymer	10	3060.0	1		synthetic construct

Sequence modifications

A: 1 - 517 (UniProt: A0LWB3)
A: 685 - 1138 (UniProt: A0LWB3)

PDB	External Database	Details
Gly 518	-	linker
Gly 680	-	linker
Gly 681	-	linker
Ser 682	-	linker
Ala 683	-	linker
Gly 684	-	linker
His 1139	-	expression tag
His 1140	-	expression tag
His 1141	-	expression tag
His 1142	-	expression tag
His 1143	-	expression tag
His 1144	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	152255.0
All*	Total formula weight	152255.0

*Water molecules are not included.