6WC0
Crystal structure of AceCas9 bound with guide RNA and DNA with 5'-NNNTC-3' PAM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | CRISPR-associated endonuclease, Csn1 family | polymer | 983 | 109589.1 | 1 | UniProt (A0LWB3) Pfam (PF18470) Pfam (PF18541) Pfam (PF17893) Pfam (PF17894) Pfam (PF18525) In PDB | Acidothermus cellulolyticus (strain ATCC 43068 / 11B) | |
| 2 | B | sgRNA (95-MER) | polymer | 94 | 30478.0 | 1 | Acidothermus cellulolyticus 11B | ||
| 3 | C | DNA (30-MER) | polymer | 30 | 9127.9 | 1 | synthetic construct | ||
| 4 | D | DNA (5'-D(*AP*TP*AP*CP*TP*TP*GP*GP*CP*G)-3') | polymer | 10 | 3060.0 | 1 | synthetic construct |
Sequence modifications
A: 1 - 517 (UniProt: A0LWB3)
A: 685 - 1138 (UniProt: A0LWB3)
A: 685 - 1138 (UniProt: A0LWB3)
| PDB | External Database | Details |
|---|---|---|
| Gly 518 | - | linker |
| Gly 680 | - | linker |
| Gly 681 | - | linker |
| Ser 682 | - | linker |
| Ala 683 | - | linker |
| Gly 684 | - | linker |
| His 1139 | - | expression tag |
| His 1140 | - | expression tag |
| His 1141 | - | expression tag |
| His 1142 | - | expression tag |
| His 1143 | - | expression tag |
| His 1144 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 152255.0 | |
| All* | Total formula weight | 152255.0 |
