6W4M

CRYSTAL STRUCTURE OF THE ADCC-POTENT, WEAKLY NEUTRALIZING HIV ENV CO-RECEPTOR BINDING SITE ANTIBODY N12-I2 FAB IN COMPLEX WITH HIV-1 CLADE A/E GP120 AND M48U1

Replaces:  5UWE

Summary for 6W4M

• Entry DOI: 10.2210/pdb6w4m/pdb
• Descriptor: clade A/E 93TH057 HIV-1 gp120 core, ANTI-HIV ANTIBODY N12-I2 FAB LIGHT CHAIN, ANTI-HIV ANTIBODY N12-I2 FAB HEAVY CHAIN, ... (5 entities in total)
• Functional Keywords: adcc and neutralizing anti-hiv-1 env antibody n12-i2, cd4i antibody, fab fragment, hiv-1 gp120, immune system, clade a/e 93th057, viral protein-immune system complex, viral protein/immune system
• Biological source: Human immunodeficiency virus 1; More
• Total number of polymer chains: 4
• Total formula weight: 93456.73

Authors

Tolbert, W.D.,Gohain, N.,Pazgier, M. (deposition date: 2020-03-11, release date: 2020-08-05, Last modification date: 2023-11-15)

Primary citation

Tolbert, W.D.,Sherburn, R.,Gohain, N.,Ding, S.,Flinko, R.,Orlandi, C.,Ray, K.,Finzi, A.,Lewis, G.K.,Pazgier, M.
Defining rules governing recognition and Fc-mediated effector functions to the HIV-1 co-receptor binding site.
Bmc Biol., 18:91-91, 2020

PubMed Abstract: The binding of HIV-1 Envelope glycoproteins (Env) to host receptor CD4 exposes vulnerable conserved epitopes within the co-receptor binding site (CoRBS) which are required for the engagement of either CCR5 or CXCR4 co-receptor to allow HIV-1 entry. Antibodies against this region have been implicated in the protection against HIV acquisition in non-human primate (NHP) challenge studies and found to act synergistically with antibodies of other specificities to deliver effective Fc-mediated effector function against HIV-1-infected cells. Here, we describe the structure and function of N12-i2, an antibody isolated from an HIV-1-infected individual, and show how the unique structural features of this antibody allow for its effective Env recognition and Fc-mediated effector function.

PubMed: 32693837
DOI: 10.1186/s12915-020-00819-y

Experimental method

X-RAY DIFFRACTION (3.2 Å)