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6TRW

Crystal structure of DPP8 in complex with the EIL peptide (SLRFLFEGQRIADNH)

Summary for 6TRW
Entry DOI10.2210/pdb6trw/pdb
DescriptorDipeptidyl peptidase 8, SER-LEU-ARG-PHE-LEU-PHE-GLU-GLY-GLN-ARG, SODIUM ION, ... (4 entities in total)
Functional Keywordseil peptide, dpp8, sumo1, hydrolase
Biological sourceHomo sapiens (Human)
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Total number of polymer chains6
Total formula weight317147.44
Authors
Ross, B.,Huber, R. (deposition date: 2019-12-19, release date: 2021-01-13, Last modification date: 2024-01-24)
Primary citationRoss, B.,Krapp, S.,Geiss-Friedlander, R.,Littmann, W.,Huber, R.,Kiefersauer, R.
Aerosol-based ligand soaking of reservoir-free protein crystals.
J.Appl.Crystallogr., 54:895-902, 2021
Cited by
PubMed Abstract: Soaking of macromolecular crystals allows the formation of complexes via diffusion of molecules into a preformed crystal for structural analysis. Soaking offers various advantages over co-crystallization, small samples and high-throughput experimentation. However, this method has disadvantages, such as inducing mechanical stress on crystals and reduced success rate caused by low affinity/solubility of the ligand. To bypass these issues, the Picodropper was previously developed in the authors' laboratory. This technique aimed to deliver small volumes of compound solution in response to crystal dehydration supported by the Free Mounting System humidity control or by IR-laser-induced protein crystal transformation. Herein, a new related soaking development, the Aerosol-Generator, is introduced. This device delivers compounds onto the solution-free surface of protein crystals using an ultrasonic technique. The produced aerosol stream enables an easier and more accurate control of solution volumes, reduced crystal handling, and crystal-size-independent soaking. The Aerosol-Generator has been used to produce complexes of DPP8 crystals, where otherwise regular soaking did not achieve complex formation. These results demonstrate the potential of this device in challenging ligand-binding scenarios and contribute to further understanding of DPP8 inhibitor binding.
PubMed: 34188616
DOI: 10.1107/S1600576721003551
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (3 Å)
Structure validation

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