6ROP
KS-MAT DI-DOMAIN OF MOUSE FAS WITH OCTANOYL COA
Summary for 6ROP
| Entry DOI | 10.2210/pdb6rop/pdb |
| Related | 5MY0 5MY2 |
| Descriptor | Fatty acid synthase, OCTANOIC ACID (CAPRYLIC ACID), OCTANOYL-COENZYME A (3 entities in total) |
| Functional Keywords | mouse fatty-acid-synthase ks-mat, transferase |
| Biological source | Mus musculus (House Mouse) |
| Total number of polymer chains | 4 |
| Total formula weight | 370355.07 |
| Authors | Paithankar, K.S.,Rittner, A.,Grininger, M. (deposition date: 2019-05-13, release date: 2019-12-04, Last modification date: 2024-11-13) |
| Primary citation | Rittner, A.,Paithankar, K.S.,Himmler, A.,Grininger, M. Type I fatty acid synthase trapped in the octanoyl-bound state. Protein Sci., 29:589-605, 2020 Cited by PubMed Abstract: De novo fatty acid biosynthesis in humans is accomplished by a multidomain protein, the Type I fatty acid synthase (FAS). Although ubiquitously expressed in all tissues, fatty acid synthesis is not essential in normal healthy cells due to sufficient supply with fatty acids by the diet. However, FAS is overexpressed in cancer cells and correlates with tumor malignancy, which makes FAS an attractive selective therapeutic target in tumorigenesis. Herein, we present a crystal structure of the condensing part of murine FAS, highly homologous to human FAS, with octanoyl moieties covalently bound to the transferase (MAT-malonyl-/acetyltransferase) and the condensation (KS-β-ketoacyl synthase) domain. The MAT domain binds the octanoyl moiety in a novel (unique) conformation, which reflects the pronounced conformational dynamics of the substrate-binding site responsible for the MAT substrate promiscuity. In contrast, the KS binding pocket just subtly adapts to the octanoyl moiety upon substrate binding. Besides the rigid domain structure, we found a positive cooperative effect in the substrate binding of the KS domain by a comprehensive enzyme kinetic study. These structural and mechanistic findings contribute significantly to our understanding of the mode of action of FAS and may guide future rational inhibitor designs. PubMed: 31811668DOI: 10.1002/pro.3797 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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