6ROP

KS-MAT DI-DOMAIN OF MOUSE FAS WITH OCTANOYL COA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	C 2 2 21
Unit cell lengths	147.440, 354.110, 218.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 2.700
R-factor	0.18688
Rwork	0.184
R-free	0.23269
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5my0
RMSD bond length	0.007
RMSD bond angle	1.544
Data reduction software	XDS (0.5.1)
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.750
High resolution limit [Å]	2.700	2.700
Rmeas	0.160
Rpim	0.040	0.600
Number of reflections	153255	7640
<I/σ(I)>	14
Completeness [%]	98.2	98.8
Redundancy	14
CC(1/2)	1.000	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	25 % w/v PEG 3350 0.2M Ammonium acteate 0.1M HEPES pH 7.5