6Q97
Structure of tmRNA SmpB bound in A site of E. coli 70S ribosome
This is a non-PDB format compatible entry.
Summary for 6Q97
| Entry DOI | 10.2210/pdb6q97/pdb |
| Related | 6Q95 |
| EMDB information | 4476 |
| Descriptor | 23S ribosomal RNA, 50S ribosomal protein L4, 50S ribosomal protein L5, ... (63 entities in total) |
| Functional Keywords | tmrna smpb trans-translation translation, ribosome |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 60 |
| Total formula weight | 2338335.08 |
| Authors | Rae, C.D. (deposition date: 2018-12-17, release date: 2019-03-13, Last modification date: 2024-04-24) |
| Primary citation | Rae, C.D.,Gordiyenko, Y.,Ramakrishnan, V. How a circularized tmRNA moves through the ribosome. Science, 363:740-744, 2019 Cited by PubMed Abstract: During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo-electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosome, enabling translation to switch from the old defective message to the reading frame on tmRNA. PubMed: 30765567DOI: 10.1126/science.aav9370 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.9 Å) |
Structure validation
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