6Q95

Structure of tmRNA SmpB bound in A site of T. thermophilus 70S ribosome

This is a non-PDB format compatible entry.

Summary for 6Q95

• Entry DOI: 10.2210/pdb6q95/pdb
• Related: 6Q97 6Q98 6Q9A
• EMDB information: 4475 4476 4477 4478
• Descriptor: 16S RIBOSOMAL RNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (62 entities in total)
• Functional Keywords: tmrna smpb trans-translation translation, ribosome
• Biological source: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579); More
• Total number of polymer chains: 61
• Total formula weight: 2359511.60

Authors

Rae, C.D. (deposition date: 2018-12-17, release date: 2019-02-27, Last modification date: 2024-05-15)

Primary citation

Rae, C.D.,Gordiyenko, Y.,Ramakrishnan, V.
How a circularized tmRNA moves through the ribosome.
Science, 363:740-744, 2019

PubMed Abstract: During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo-electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosome, enabling translation to switch from the old defective message to the reading frame on tmRNA.

PubMed: 30765567
DOI: 10.1126/science.aav9370

Experimental method

ELECTRON MICROSCOPY (3.7 Å)