6PIS

Mouse two pore domain K+ channel TRAAK (K2P4.1) - Fab complex structure

Summary for 6PIS

• Entry DOI: 10.2210/pdb6pis/pdb
• Descriptor: Potassium channel subfamily K member 4, ANTIBODY FAB FRAGMENT LIGHT CHAIN, ANTIBODY FAB FRAGMENT HEAVY CHAIN, ... (4 entities in total)
• Functional Keywords: potassium ion channel, ion transport, membrane protein
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 6
• Total formula weight: 162810.46

Authors

Brohawn, S.G. (deposition date: 2019-06-27, release date: 2019-12-25, Last modification date: 2024-10-23)

Primary citation

Brohawn, S.G.,Wang, W.,Handler, A.,Campbell, E.B.,Schwarz, J.R.,MacKinnon, R.
The mechanosensitive ion channel TRAAK is localized to the mammalian node of Ranvier.
Elife, 8:-, 2019

PubMed Abstract: TRAAK is a membrane tension-activated K channel that has been associated through behavioral studies to mechanical nociception. We used specific monoclonal antibodies in mice to show that TRAAK is localized exclusively to nodes of Ranvier, the action potential propagating elements of myelinated nerve fibers. Approximately 80 percent of myelinated nerve fibers throughout the central and peripheral nervous system contain TRAAK in what is likely an all-nodes or no-nodes per axon fashion. TRAAK is not observed at the axon initial segment where action potentials are first generated. We used polyclonal antibodies, the TRAAK inhibitor RU2 and node clamp amplifiers to demonstrate the presence and functional properties of TRAAK in rat nerve fibers. TRAAK contributes to the 'leak' K current in mammalian nerve fiber conduction by hyperpolarizing the resting membrane potential, thereby increasing Na channel availability for action potential propagation. We speculate on why nodes of Ranvier contain a mechanosensitive K channel.

PubMed: 31674909
DOI: 10.7554/eLife.50403

Experimental method

X-RAY DIFFRACTION (2.77 Å)