6PIS

Mouse two pore domain K+ channel TRAAK (K2P4.1) - Fab complex structure

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Potassium channel subfamily K member 4	polymer	310	33725.9	2	UniProt (O88454) Pfam (PF07885)	Mus musculus (Mouse)	TWIK-related arachidonic acid-stimulated potassium channel protein,TRAAK
2	C, F (L, M)	ANTIBODY FAB FRAGMENT LIGHT CHAIN	polymer	213	23526.2	2		Cricetulus migratorius
3	D, E (H, I)	ANTIBODY FAB FRAGMENT HEAVY CHAIN	polymer	224	24035.9	2		Cricetulus migratorius
4	G, H, I, J, K... (A, B)	POTASSIUM ION	non-polymer		39.1	6	Chemie (K)

Sequence modifications

A, B: 27 - 301 (UniProt: O88454)

PDB	External Database	Details
Met 1	-	expression tag
Thr 2	-	expression tag
Thr 3	-	expression tag
Ala 4	-	expression tag
Pro 5	-	expression tag
Gln 6	-	expression tag
Glu 7	-	expression tag
Pro 8	-	expression tag
Pro 9	-	expression tag
Ala 10	-	expression tag
Arg 11	-	expression tag
Pro 12	-	expression tag
Leu 13	-	expression tag
Gln 14	-	expression tag
Ala 15	-	expression tag
Gly 16	-	expression tag
Ser 17	-	expression tag
Gly 18	-	expression tag
Ala 19	-	expression tag
Gly 20	-	expression tag
Pro 21	-	expression tag
Ala 22	-	expression tag
Pro 23	-	expression tag
Gly 24	-	expression tag
Arg 25	-	expression tag
Ala 26	-	expression tag
Gln 107	Asn 81	engineered mutation
Gln 110	Asn 84	engineered mutation
Ser 302	-	expression tag
Asn 303	-	expression tag
Ser 304	-	expression tag
Leu 305	-	expression tag
Glu 306	-	expression tag
Val 307	-	expression tag
Leu 308	-	expression tag
Phe 309	-	expression tag
Gln 310	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	162575.9
Non-Polymers*	Number of molecules	6
	Total formula weight	234.6
All*	Total formula weight	162810.5

*Water molecules are not included.