6PIS

Mouse two pore domain K+ channel TRAAK (K2P4.1) - Fab complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	72.450, 154.510, 202.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	122.910 - 2.770
R-factor	0.2503
Rwork	0.248
R-free	0.28630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wfe
RMSD bond length	0.006
RMSD bond angle	0.923
Data reduction software	XDS (March 15, 2019)
Data scaling software	XSCALE (March 15, 2019)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	122.910	122.910	2.670
High resolution limit [Å]	2.600	11.620	2.600
Rmerge	0.483	0.071
Rmeas	0.502	0.074
Number of reflections	26117	900	4643
<I/σ(I)>	3.49	25.72
Completeness [%]	98.7	98	89.4
Redundancy	13.384	11.751	5.068
CC(1/2)	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	50 mM Sodium Citrate pH 5.5 500 mM KCl 12% PEG4000 (w/v)