6NF5

BG505 MD64 N332-GT5 SOSIP trimer in complex with BG18-like precursor HMP1 fragmentantigen binding and base-binding RM20A3 fragment antigen binding

Summary for 6NF5

• Entry DOI: 10.2210/pdb6nf5/pdb
• EMDB information: 7884
• Descriptor: HIV-1 Env BG505 MD64 N332-GT5 SOSIP gp120, 2-acetamido-2-deoxy-beta-D-glucopyranose, HIV-1 Env BG505 MD64 N332-GT5 SOSIP gp41, ... (10 entities in total)
• Functional Keywords: hiv-1, hiv envelope, sosip, antibody, trimer, precursor antibody, viral protein, viral protein-immune system complex, viral protein/immune system
• Biological source: Human immunodeficiency virus 1; More
• Total number of polymer chains: 18
• Total formula weight: 394354.09

Authors

Ozorowski, G.,Torres, J.L.,Steichen, J.M.,Schief, W.R.,Ward, A.B. (deposition date: 2018-12-18, release date: 2019-11-06, Last modification date: 2024-11-06)

Primary citation

Steichen, J.M.,Lin, Y.C.,Havenar-Daughton, C.,Pecetta, S.,Ozorowski, G.,Willis, J.R.,Toy, L.,Sok, D.,Liguori, A.,Kratochvil, S.,Torres, J.L.,Kalyuzhniy, O.,Melzi, E.,Kulp, D.W.,Raemisch, S.,Hu, X.,Bernard, S.M.,Georgeson, E.,Phelps, N.,Adachi, Y.,Kubitz, M.,Landais, E.,Umotoy, J.,Robinson, A.,Briney, B.,Wilson, I.A.,Burton, D.R.,Ward, A.B.,Crotty, S.,Batista, F.D.,Schief, W.R.
A generalized HIV vaccine design strategy for priming of broadly neutralizing antibody responses.
Science, 366:-, 2019

PubMed Abstract: Vaccine induction of broadly neutralizing antibodies (bnAbs) to HIV remains a major challenge. Germline-targeting immunogens hold promise for initiating the induction of certain bnAb classes; yet for most bnAbs, a strong dependence on antibody heavy chain complementarity-determining region 3 (HCDR3) is a major barrier. Exploiting ultradeep human antibody sequencing data, we identified a diverse set of potential antibody precursors for a bnAb with dominant HCDR3 contacts. We then developed HIV envelope trimer-based immunogens that primed responses from rare bnAb-precursor B cells in a mouse model and bound a range of potential bnAb-precursor human naïve B cells in ex vivo screens. Our repertoire-guided germline-targeting approach provides a framework for priming the induction of many HIV bnAbs and could be applied to most HCDR3-dominant antibodies from other pathogens.

PubMed: 31672916
DOI: 10.1126/science.aax4380

Experimental method

ELECTRON MICROSCOPY (3.71 Å)