6MN5

Crystal structure of aminoglycoside acetyltransferase AAC(3)-IVa, H154A mutant, in complex with gentamicin C1A

Summary for 6MN5

• Entry DOI: 10.2210/pdb6mn5/pdb
• Descriptor: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa, (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL, ZINC ION, ... (8 entities in total)
• Functional Keywords: antibiotic_nat family, acetyltransferase, aminoglycoside, antibiotic resistance, coenzyme a, gentamicin, aac(3)-iva, csgid, transferase, center for structural genomics of infectious diseases, national institute of allergy and infectious diseases, niaid, transferase-antibiotic complex, transferase/antibiotic
• Biological source: Escherichia coli
• Total number of polymer chains: 6
• Total formula weight: 180757.24

Authors

Stogios, P.J.,Evdokimova, E.,Kim, Y.,Di Leo, R.,Savchenko, A.,Joachimiak, A.,Satchell, K.J.,Center for Structural Genomics of Infectious Diseases (CSGID) (deposition date: 2018-10-01, release date: 2018-10-24, Last modification date: 2023-10-11)

• Primary citation: Stogios, P.J.,Bordeleau, E.,Xu, Z.,Skarina, T.,Evdokimova, E.,Chou, S.,Diorio-Toth, L.,D'Souza, A.W.,Patel, S.,Dantas, G.,Wright, G.D.,Savchenko, A.; Structural and molecular rationale for the diversification of resistance mediated by the Antibiotic_NAT family.; Commun Biol, 5:263-263, 2022; PubMed: 35338238; DOI: 10.1038/s42003-022-03219-w

Experimental method

X-RAY DIFFRACTION (2.58 Å)