6MN5
Crystal structure of aminoglycoside acetyltransferase AAC(3)-IVa, H154A mutant, in complex with gentamicin C1A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa | polymer | 262 | 28316.4 | 6 | UniProt (Q306W4) Pfam (PF02522) | Escherichia coli | |
| 2 | G, KA, OB, W, WB... (A, C, E, B, F...) | (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL | non-polymer | 449.5 | 6 | Chemie (LLL) | |||
| 3 | AB, H, LA, PB, X... (D, A, C, E, B...) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) | |||
| 4 | BB, CB, I, J, MA... (D, A, C, E, B...) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 12 | Chemie (EPE) | |||
| 5 | AA, DB, EB, K, OA (B, D, A, C) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 6 | AC, BA, CA, DA, EA... (F, B, D, A, C...) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 41 | Chemie (EDO) | |||
| 7 | JA, NB, YA (B, D, C) | 3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL | non-polymer | 634.8 | 3 | Chemie (PE3) | |||
| 8 | BC, CC, DC, EC, FC... (A, B, C, D, E...) | water | water | 18.0 | 509 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 1 - 258 (UniProt: Q306W4)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Met 0 | - | expression tag |
| Ala 154 | His 154 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 169898.4 | |
| Non-Polymers* | Number of molecules | 73 |
| Total formula weight | 10858.9 | |
| All* | Total formula weight | 180757.2 |
