6MFT

Crystal structure of glycosylated 426c HIV-1 gp120 core G459C in complex with glVRC01 A60C heavy chain

Summary for 6MFT

• Entry DOI: 10.2210/pdb6mft/pdb
• Descriptor: Heavy Chain glVRC01, 1,2-ETHANEDIOL, Light chain glVRC01, ... (11 entities in total)
• Functional Keywords: glycans, germline, immune system-viral protein complex, immune system/viral protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 6
• Total formula weight: 180022.22

Authors

Weidle, C.,Pancera, M.,Stamatatos, L.,Gray, M. (deposition date: 2018-09-12, release date: 2018-11-14, Last modification date: 2024-10-23)

Primary citation

Borst, A.J.,Weidle, C.E.,Gray, M.D.,Frenz, B.,Snijder, J.,Joyce, M.G.,Georgiev, I.S.,Stewart-Jones, G.B.,Kwong, P.D.,McGuire, A.T.,DiMaio, F.,Stamatatos, L.,Pancera, M.,Veesler, D.
Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.
Elife, 7:-, 2018

PubMed Abstract: VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env). Unlike mature antibodies, corresponding VRC01 germline precursors poorly bind to Env. Immunogen design has mostly relied on glycan removal from trimeric Env constructs and has had limited success in eliciting mature VRC01 bnAbs. To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics. Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former. Interactions in the presence of an Asn276 oligosaccharide could be enhanced upon carbohydrate shortening, which should be considered for immunogen design.

PubMed: 30403372
DOI: 10.7554/eLife.37688

Experimental method

X-RAY DIFFRACTION (2.315 Å)