6MFT

Crystal structure of glycosylated 426c HIV-1 gp120 core G459C in complex with glVRC01 A60C heavy chain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	197.082, 109.003, 103.225
Unit cell angles	90.00, 114.47, 90.00

Refinement procedure

Resolution	49.152 - 2.315
R-factor	0.2464
Rwork	0.244
R-free	0.29510
RMSD bond length	0.003
RMSD bond angle	0.588
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.315	2.315
Number of reflections	83087
<I/σ(I)>	23.4
Completeness [%]	95.6
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.09M MgCl2, 0.09M Na Citrate pH 5.0, 13.5% PEG 4K, 0.1M LiCl2