6KC7

Crystal structure of Nme1Cas9 in complex with sgRNA and target DNA (ATATGATT PAM) in seed-base paring state

Summary for 6KC7

• Entry DOI: 10.2210/pdb6kc7/pdb
• Descriptor: CRISPR-associated endonuclease Cas9, sgRNA, DNA (5'-D(*TP*AP*AP*AP*AP*TP*CP*AP*TP*AP*TP*GP*TP*AP*AP*AP*GP*TP*T)-3'), ... (4 entities in total)
• Functional Keywords: crispr-cas9, nmecas9, nme1cas9, hydrolase, ternary complex, hydrolase-rna-dna complex, hydrolase/rna/dna
• Biological source: Neisseria meningitidis 8013; More
• Total number of polymer chains: 4
• Total formula weight: 176906.33

Authors

Sun, W.,Yang, J.,Cheng, Z.,Liu, C.,Wang, K.,Huang, X.,Wang, Y. (deposition date: 2019-06-27, release date: 2019-11-06, Last modification date: 2023-11-22)

• Primary citation: Sun, W.,Yang, J.,Cheng, Z.,Amrani, N.,Liu, C.,Wang, K.,Ibraheim, R.,Edraki, A.,Huang, X.,Wang, M.,Wang, J.,Liu, L.,Sheng, G.,Yang, Y.,Lou, J.,Sontheimer, E.J.,Wang, Y.; Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States.; Mol.Cell, 76:938-, 2019; PubMed: 31668930; DOI: 10.1016/j.molcel.2019.09.025

Experimental method

X-RAY DIFFRACTION (3.3 Å)