6KC7
Crystal structure of Nme1Cas9 in complex with sgRNA and target DNA (ATATGATT PAM) in seed-base paring state
Summary for 6KC7
Entry DOI | 10.2210/pdb6kc7/pdb |
Descriptor | CRISPR-associated endonuclease Cas9, sgRNA, DNA (5'-D(*TP*AP*AP*AP*AP*TP*CP*AP*TP*AP*TP*GP*TP*AP*AP*AP*GP*TP*T)-3'), ... (4 entities in total) |
Functional Keywords | crispr-cas9, nmecas9, nme1cas9, hydrolase, ternary complex, hydrolase-rna-dna complex, hydrolase/rna/dna |
Biological source | Neisseria meningitidis 8013 More |
Total number of polymer chains | 4 |
Total formula weight | 176906.33 |
Authors | |
Primary citation | Sun, W.,Yang, J.,Cheng, Z.,Amrani, N.,Liu, C.,Wang, K.,Ibraheim, R.,Edraki, A.,Huang, X.,Wang, M.,Wang, J.,Liu, L.,Sheng, G.,Yang, Y.,Lou, J.,Sontheimer, E.J.,Wang, Y. Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States. Mol.Cell, 76:938-, 2019 Cited by PubMed: 31668930DOI: 10.1016/j.molcel.2019.09.025 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3.3 Å) |
Structure validation
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