6KC7
Crystal structure of Nme1Cas9 in complex with sgRNA and target DNA (ATATGATT PAM) in seed-base paring state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | CRISPR-associated endonuclease Cas9 | polymer | 1083 | 124633.9 | 1 | UniProt (C9X1G5) Pfam (PF13395) Pfam (PF18541) In PDB | Neisseria meningitidis 8013 | |
| 2 | B | sgRNA | polymer | 135 | 43059.4 | 1 | synthetic construct | ||
| 3 | C | DNA (5'-D(*TP*AP*AP*AP*AP*TP*CP*AP*TP*AP*TP*GP*TP*AP*AP*AP*GP*TP*T)-3') | polymer | 19 | 5850.8 | 1 | synthetic construct | ||
| 4 | D | DNA (5'-D(*AP*TP*AP*TP*GP*AP*TP*TP*TP*TP*A)-3') | polymer | 11 | 3362.2 | 1 | synthetic construct |
Sequence modifications
A: 1 - 1082 (UniProt: C9X1G5)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Ala 588 | His 588 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 176906.3 | |
| All* | Total formula weight | 176906.3 |
