6KC7
Crystal structure of Nme1Cas9 in complex with sgRNA and target DNA (ATATGATT PAM) in seed-base paring state
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003676 | molecular_function | nucleic acid binding |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0043571 | biological_process | maintenance of CRISPR repeat elements |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051607 | biological_process | defense response to virus |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: For RuvC-like nuclease domain => ECO:0000255|HAMAP-Rule:MF_01480 |
| Chain | Residue | Details |
| A | ASP16 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton acceptor for HNH nuclease domain => ECO:0000255|HAMAP-Rule:MF_01480 |
| Chain | Residue | Details |
| A | ALA588 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01480 |
| Chain | Residue | Details |
| A | ASP16 | |
| A | GLU504 | |
| A | GLU508 | |
| A | HIS723 |
