6IAO
Structure of Cytochrome P450 BM3 M11 mutant in complex with DTT at resolution 2.16A
Summary for 6IAO
| Entry DOI | 10.2210/pdb6iao/pdb |
| Descriptor | Bifunctional cytochrome P450/NADPH--P450 reductase, PROTOPORPHYRIN IX CONTAINING FE, 2,3-DIHYDROXY-1,4-DITHIOBUTANE, ... (7 entities in total) |
| Functional Keywords | cytochrome p450 bm3, mutant m11, dtt, oxidoreductase |
| Biological source | Bacillus megaterium |
| Total number of polymer chains | 4 |
| Total formula weight | 233804.46 |
| Authors | Mirza, O.,Rafiq, M.,Frydenvang, K. (deposition date: 2018-11-27, release date: 2019-06-05, Last modification date: 2024-10-23) |
| Primary citation | Frydenvang, K.,Verkade-Vreeker, M.C.A.,Dohmen, F.,Commandeur, J.N.M.,Rafiq, M.,Mirza, O.,Jorgensen, F.S.,Geerke, D.P. Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol. Plos One, 14:e0217292-e0217292, 2019 Cited by PubMed Abstract: The bacterial Cytochrome P450 (CYP) BM3 (CYP102A1) is one of the most active CYP isoforms. BM3 mutants can serve as a model for human drug-metabolizing CYPs and/or as biocatalyst for selective formation of drug metabolites. Hence, molecular and computational biologists have in the last two decades shown strong interest in the discovery and design of novel BM3 variants with optimized activity and selectivity for substrate conversion. This led e.g. to the discovery of mutant M11 that is able to metabolize a variety of drugs and drug-like compounds with relatively high activity. In order to further improve our understanding of CYP binding and reactions, we performed a co-crystallization study of mutant M11 and report here the three-dimensional structure M11 in complex with dithiothreitol (DTT) at a resolution of 2.16 Å. The structure shows that DTT can coordinate to the Fe atom in the heme group. UV/Vis spectroscopy and molecular dynamics simulation studies underline this finding and as first structure of the CYP BM3 mutant M11 in complex with a ligand, it offers a basis for structure-based design of novel mutants. PubMed: 31125381DOI: 10.1371/journal.pone.0217292 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.16 Å) |
Structure validation
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