6IAO
Structure of Cytochrome P450 BM3 M11 mutant in complex with DTT at resolution 2.16A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | Bifunctional cytochrome P450/NADPH--P450 reductase | polymer | 507 | 57319.4 | 4 | UniProt (P14779) Pfam (PF00067) In PDB | Bacillus megaterium | Cytochrome P450(BM-3),Cytochrome P450BM-3,Fatty acid monooxygenase,Flavocytochrome P450 BM3 |
| 2 | A, D, B, C | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 4 | Chemie (HEM) | |||
| 3 | A, D, B, C | 2,3-DIHYDROXY-1,4-DITHIOBUTANE | non-polymer | 154.3 | 5 | Chemie (DTT) | |||
| 4 | C, A, D, B | CHLORIDE ION | non-polymer | 35.5 | 20 | Chemie (CL) | |||
| 5 | C, A, B | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 6 | C, A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
| 7 | water | water | 18.0 | 1245 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 0 - 472 (UniProt: P14779)
| PDB | External Database | Details |
|---|---|---|
| Met -34 | - | initiating methionine |
| Gly -33 | - | expression tag |
| Ser -32 | - | expression tag |
| Ser -31 | - | expression tag |
| His -30 | - | expression tag |
| His -29 | - | expression tag |
| His -28 | - | expression tag |
| His -27 | - | expression tag |
| His -26 | - | expression tag |
| His -25 | - | expression tag |
| Ser -24 | - | expression tag |
| Ser -23 | - | expression tag |
| Gly -22 | - | expression tag |
| Leu -21 | - | expression tag |
| Val -20 | - | expression tag |
| Pro -19 | - | expression tag |
| Arg -18 | - | expression tag |
| Gly -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| Met -14 | - | expression tag |
| Ala -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Met -11 | - | expression tag |
| Thr -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Gln -7 | - | expression tag |
| Gln -6 | - | expression tag |
| Met -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Leu 47 | Arg 48 | engineered mutation |
| Gly 64 | Glu 65 | engineered mutation |
| Ile 81 | Phe 82 | engineered mutation |
| Val 87 | Phe 88 | engineered mutation |
| Gly 143 | Glu 144 | engineered mutation |
| Gln 188 | Leu 189 | engineered mutation |
| Cys 198 | Tyr 199 | engineered mutation |
| Val 267 | Glu 268 | engineered mutation |
| Tyr 285 | His 286 | engineered mutation |
| Ser 415 | Gly 416 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 229277.6 | |
| Non-Polymers* | Number of molecules | 35 |
| Total formula weight | 4526.9 | |
| All* | Total formula weight | 233804.5 |
