6IAN
T. brucei IFT22/74/81 GTP-bound crystal structure
Summary for 6IAN
| Entry DOI | 10.2210/pdb6ian/pdb |
| Descriptor | Intraflagellar transport protein 74, Intraflagellar transport protein 81, Rab-like 5, ... (6 entities in total) |
| Functional Keywords | intraflagellar transport protein 22, ift74, ift81, cilium formation, rab-like, gtpase, rabl5, cytosolic protein |
| Biological source | Trypanosoma brucei brucei (strain 927/4 GUTat10.1) More |
| Total number of polymer chains | 5 |
| Total formula weight | 206556.58 |
| Authors | Wachter, S.,Basquin, J.,Lorentzen, E. (deposition date: 2018-11-27, release date: 2019-05-01, Last modification date: 2025-10-01) |
| Primary citation | Wachter, S.,Jung, J.,Shafiq, S.,Basquin, J.,Fort, C.,Bastin, P.,Lorentzen, E. Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly. Embo J., 38:-, 2019 Cited by PubMed Abstract: Intraflagellar transport (IFT) relies on motor proteins and the IFT complex to construct cilia and flagella. The IFT complex subunit IFT22/RabL5 has sequence similarity with small GTPases although the nucleotide specificity is unclear because of non-conserved G4/G5 motifs. We show that IFT22 specifically associates with G-nucleotides and present crystal structures of IFT22 in complex with GDP, GTP, and with IFT74/81. Our structural analysis unravels an unusual GTP/GDP-binding mode of IFT22 bypassing the classical G4 motif. The GTPase switch regions of IFT22 become ordered upon complex formation with IFT74/81 and mediate most of the IFT22-74/81 interactions. Structure-based mutagenesis reveals that association of IFT22 with the IFT complex is essential for flagellum construction in although IFT22 GTP-loading is not strictly required. PubMed: 30940671DOI: 10.15252/embj.2018101251 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.2 Å) |
Structure validation
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