6IAN
T. brucei IFT22/74/81 GTP-bound crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97899 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.860, 228.300, 115.710 |
| Unit cell angles | 90.00, 96.76, 90.00 |
Refinement procedure
| Resolution | 80.982 - 3.200 |
| R-factor | 0.2431 |
| Rwork | 0.241 |
| R-free | 0.27970 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.058 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.000 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.210 | 4.110 |
| Number of reflections | 113747 | 18991 |
| <I/σ(I)> | 11.5 | 0.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.3 | |
| CC(1/2) | 0.999 | 0.395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 15% (v/v) glycerol, 7.5% (w/v) PEG4000, 100 mM HEPES pH 7.5 |
