6IAN

T. brucei IFT22/74/81 GTP-bound crystal structure

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Intraflagellar transport protein 74	polymer	328	37731.6	2	UniProt (Q57WF2) In PDB	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
2	C, D	Intraflagellar transport protein 81	polymer	454	52904.8	2	UniProt (Q38BY1) Pfam (PF18383) In PDB	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
3	E	Rab-like 5	polymer	220	24736.3	1	UniProt (A0A3L6KZ98) Pfam (PF08477) UniProt (by SIFTS) (Q381A3) In PDB	Trypanosoma brucei equiperdum
4	E	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
5	E	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6		water	water		18.0	46	Chemie (HOH)

Sequence modifications

A, B: 79 - 401 (UniProt: Q57WF2)

PDB	External Database	Details
Gly 74	-	expression tag
Ala 75	-	expression tag
Ala 76	-	expression tag
Ser 77	-	expression tag
Mse 78	-	expression tag
Ala 199	Thr 193	conflict

C, D: 2 - 450 (UniProt: Q38BY1)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Mse 1	-	expression tag

E: 2 - 220 (UniProt: A0A3L6KZ98)

PDB	External Database	Details
Mse 1	-	initiating methionine
Pro 161	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	206009.1
Non-Polymers*	Number of molecules	2
	Total formula weight	547.5
All*	Total formula weight	206556.6

*Water molecules are not included.