6I00
Cryo-EM informed directed evolution of Nitrilase 4 leads to a change in quaternary structure.
Summary for 6I00
| Entry DOI | 10.2210/pdb6i00/pdb |
| EMDB information | 0320 |
| Descriptor | Bifunctional nitrilase/nitrile hydratase NIT4 (1 entity in total) |
| Functional Keywords | nitrilase, cyanide detoxification, beta-cyano-l-alanine hydrolase, helical filament, hydrolase |
| Biological source | Arabidopsis thaliana (Mouse-ear cress) |
| Total number of polymer chains | 12 |
| Total formula weight | 477193.36 |
| Authors | Mulelu, A.E.,Woodward, J.D. (deposition date: 2018-10-24, release date: 2019-07-24, Last modification date: 2024-05-15) |
| Primary citation | Mulelu, A.E.,Kirykowicz, A.M.,Woodward, J.D. Cryo-EM and directed evolution reveal howArabidopsisnitrilase specificity is influenced by its quaternary structure. Commun Biol, 2:260-260, 2019 Cited by PubMed: 31341959DOI: 10.1038/s42003-019-0505-4 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.4 Å) |
Structure validation
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