6I00

Cryo-EM informed directed evolution of Nitrilase 4 leads to a change in quaternary structure.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000257	molecular_function	nitrilase activity
A	0003824	molecular_function	catalytic activity
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0016787	molecular_function	hydrolase activity
A	0016829	molecular_function	lyase activity
A	0016836	molecular_function	hydro-lyase activity
A	0018822	molecular_function	nitrile hydratase activity
A	0019499	biological_process	cyanide metabolic process
A	0047427	molecular_function	cyanoalanine nitrilase activity
A	0047558	molecular_function	3-cyanoalanine hydratase activity
A	0051410	biological_process	detoxification of nitrogen compound
A	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
B	0000257	molecular_function	nitrilase activity
B	0003824	molecular_function	catalytic activity
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0016787	molecular_function	hydrolase activity
B	0016829	molecular_function	lyase activity
B	0016836	molecular_function	hydro-lyase activity
B	0018822	molecular_function	nitrile hydratase activity
B	0019499	biological_process	cyanide metabolic process
B	0047427	molecular_function	cyanoalanine nitrilase activity
B	0047558	molecular_function	3-cyanoalanine hydratase activity
B	0051410	biological_process	detoxification of nitrogen compound
B	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
C	0000257	molecular_function	nitrilase activity
C	0003824	molecular_function	catalytic activity
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0016787	molecular_function	hydrolase activity
C	0016829	molecular_function	lyase activity
C	0016836	molecular_function	hydro-lyase activity
C	0018822	molecular_function	nitrile hydratase activity
C	0019499	biological_process	cyanide metabolic process
C	0047427	molecular_function	cyanoalanine nitrilase activity
C	0047558	molecular_function	3-cyanoalanine hydratase activity
C	0051410	biological_process	detoxification of nitrogen compound
C	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
D	0000257	molecular_function	nitrilase activity
D	0003824	molecular_function	catalytic activity
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0016787	molecular_function	hydrolase activity
D	0016829	molecular_function	lyase activity
D	0016836	molecular_function	hydro-lyase activity
D	0018822	molecular_function	nitrile hydratase activity
D	0019499	biological_process	cyanide metabolic process
D	0047427	molecular_function	cyanoalanine nitrilase activity
D	0047558	molecular_function	3-cyanoalanine hydratase activity
D	0051410	biological_process	detoxification of nitrogen compound
D	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
E	0000257	molecular_function	nitrilase activity
E	0003824	molecular_function	catalytic activity
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0016787	molecular_function	hydrolase activity
E	0016829	molecular_function	lyase activity
E	0016836	molecular_function	hydro-lyase activity
E	0018822	molecular_function	nitrile hydratase activity
E	0019499	biological_process	cyanide metabolic process
E	0047427	molecular_function	cyanoalanine nitrilase activity
E	0047558	molecular_function	3-cyanoalanine hydratase activity
E	0051410	biological_process	detoxification of nitrogen compound
E	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
F	0000257	molecular_function	nitrilase activity
F	0003824	molecular_function	catalytic activity
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0016787	molecular_function	hydrolase activity
F	0016829	molecular_function	lyase activity
F	0016836	molecular_function	hydro-lyase activity
F	0018822	molecular_function	nitrile hydratase activity
F	0019499	biological_process	cyanide metabolic process
F	0047427	molecular_function	cyanoalanine nitrilase activity
F	0047558	molecular_function	3-cyanoalanine hydratase activity
F	0051410	biological_process	detoxification of nitrogen compound
F	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
G	0000257	molecular_function	nitrilase activity
G	0003824	molecular_function	catalytic activity
G	0005829	cellular_component	cytosol
G	0005886	cellular_component	plasma membrane
G	0016787	molecular_function	hydrolase activity
G	0016829	molecular_function	lyase activity
G	0016836	molecular_function	hydro-lyase activity
G	0018822	molecular_function	nitrile hydratase activity
G	0019499	biological_process	cyanide metabolic process
G	0047427	molecular_function	cyanoalanine nitrilase activity
G	0047558	molecular_function	3-cyanoalanine hydratase activity
G	0051410	biological_process	detoxification of nitrogen compound
G	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
H	0000257	molecular_function	nitrilase activity
H	0003824	molecular_function	catalytic activity
H	0005829	cellular_component	cytosol
H	0005886	cellular_component	plasma membrane
H	0016787	molecular_function	hydrolase activity
H	0016829	molecular_function	lyase activity
H	0016836	molecular_function	hydro-lyase activity
H	0018822	molecular_function	nitrile hydratase activity
H	0019499	biological_process	cyanide metabolic process
H	0047427	molecular_function	cyanoalanine nitrilase activity
H	0047558	molecular_function	3-cyanoalanine hydratase activity
H	0051410	biological_process	detoxification of nitrogen compound
H	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
I	0000257	molecular_function	nitrilase activity
I	0003824	molecular_function	catalytic activity
I	0005829	cellular_component	cytosol
I	0005886	cellular_component	plasma membrane
I	0016787	molecular_function	hydrolase activity
I	0016829	molecular_function	lyase activity
I	0016836	molecular_function	hydro-lyase activity
I	0018822	molecular_function	nitrile hydratase activity
I	0019499	biological_process	cyanide metabolic process
I	0047427	molecular_function	cyanoalanine nitrilase activity
I	0047558	molecular_function	3-cyanoalanine hydratase activity
I	0051410	biological_process	detoxification of nitrogen compound
I	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
J	0000257	molecular_function	nitrilase activity
J	0003824	molecular_function	catalytic activity
J	0005829	cellular_component	cytosol
J	0005886	cellular_component	plasma membrane
J	0016787	molecular_function	hydrolase activity
J	0016829	molecular_function	lyase activity
J	0016836	molecular_function	hydro-lyase activity
J	0018822	molecular_function	nitrile hydratase activity
J	0019499	biological_process	cyanide metabolic process
J	0047427	molecular_function	cyanoalanine nitrilase activity
J	0047558	molecular_function	3-cyanoalanine hydratase activity
J	0051410	biological_process	detoxification of nitrogen compound
J	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
K	0000257	molecular_function	nitrilase activity
K	0003824	molecular_function	catalytic activity
K	0005829	cellular_component	cytosol
K	0005886	cellular_component	plasma membrane
K	0016787	molecular_function	hydrolase activity
K	0016829	molecular_function	lyase activity
K	0016836	molecular_function	hydro-lyase activity
K	0018822	molecular_function	nitrile hydratase activity
K	0019499	biological_process	cyanide metabolic process
K	0047427	molecular_function	cyanoalanine nitrilase activity
K	0047558	molecular_function	3-cyanoalanine hydratase activity
K	0051410	biological_process	detoxification of nitrogen compound
K	0080061	molecular_function	indole-3-acetonitrile nitrilase activity
L	0000257	molecular_function	nitrilase activity
L	0003824	molecular_function	catalytic activity
L	0005829	cellular_component	cytosol
L	0005886	cellular_component	plasma membrane
L	0016787	molecular_function	hydrolase activity
L	0016829	molecular_function	lyase activity
L	0016836	molecular_function	hydro-lyase activity
L	0018822	molecular_function	nitrile hydratase activity
L	0019499	biological_process	cyanide metabolic process
L	0047427	molecular_function	cyanoalanine nitrilase activity
L	0047558	molecular_function	3-cyanoalanine hydratase activity
L	0051410	biological_process	detoxification of nitrogen compound
L	0080061	molecular_function	indole-3-acetonitrile nitrilase activity

Functional Information from PROSITE/UniProt

site_id	PS00920
Number of Residues	16
Details	NITRIL_CHT_1 Nitrilases / cyanide hydratase signature 1. GsqLVvFpEafIgGYP

Chain	Residue	Details
J	GLY68-PRO83

---

site_id	PS00921
Number of Residues	14
Details	NITRIL_CHT_2 Nitrilases / cyanide hydratase active site signature. GAaiCWENrmPSlR

Chain	Residue	Details
J	GLY193-ARG206

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU00054

Chain	Residue	Details
J	GLU76
A	GLU76
L	GLU76
K	GLU76
I	GLU76
H	GLU76
G	GLU76
F	GLU76
E	GLU76
D	GLU76
C	GLU76
B	GLU76

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	ACT_SITE: Proton donor => ECO:0000255|PROSITE-ProRule:PRU00054

Chain	Residue	Details
J	LYS163
A	LYS163
L	LYS163
K	LYS163
I	LYS163
H	LYS163
G	LYS163
F	LYS163
E	LYS163
D	LYS163
C	LYS163
B	LYS163

---

site_id	SWS_FT_FI3
Number of Residues	12
Details	ACT_SITE: Nucleophile => ECO:0000255|PROSITE-ProRule:PRU00054, ECO:0000255|PROSITE-ProRule:PRU10105

Chain	Residue	Details
J	CYS197
A	CYS197
L	CYS197
K	CYS197
I	CYS197
H	CYS197
G	CYS197
F	CYS197
E	CYS197
D	CYS197
C	CYS197
B	CYS197

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	MOD_RES: N-acetylserine => ECO:0000250|UniProtKB:P32962

Chain	Residue	Details
J	SER2
A	SER2
L	SER2
K	SER2
I	SER2
H	SER2
G	SER2
F	SER2
E	SER2
D	SER2
C	SER2
B	SER2

---