6HQA

Molecular structure of promoter-bound yeast TFIID

Summary for 6HQA

• Entry DOI: 10.2210/pdb6hqa/pdb
• EMDB information: 0251
• Descriptor: Taf2, Subunit (90 kDa) of TFIID and SAGA complexes, Subunit (60 kDa) of TFIID and SAGA complexes, ... (9 entities in total)
• Functional Keywords: complex, transcription initiation, transcription
• Biological source: Komagataella phaffii GS115; More
• Total number of polymer chains: 11
• Total formula weight: 634429.33

Authors

Kolesnikova, O.,Ben-Shem, A.,Luo, J.,Ranish, J.,Schultz, P.,Papai, G. (deposition date: 2018-09-24, release date: 2018-11-21, Last modification date: 2024-10-23)

Primary citation

Kolesnikova, O.,Ben-Shem, A.,Luo, J.,Ranish, J.,Schultz, P.,Papai, G.
Molecular structure of promoter-bound yeast TFIID.
Nat Commun, 9:4666-4666, 2018

PubMed Abstract: Transcription preinitiation complex assembly on the promoters of protein encoding genes is nucleated in vivo by TFIID composed of the TATA-box Binding Protein (TBP) and 13 TBP-associate factors (Tafs) providing regulatory and chromatin binding functions. Here we present the cryo-electron microscopy structure of promoter-bound yeast TFIID at a resolution better than 5 Å, except for a flexible domain. We position the crystal structures of several subunits and, in combination with cross-linking studies, describe the quaternary organization of TFIID. The compact tri lobed architecture is stabilized by a topologically closed Taf5-Taf6 tetramer. We confirm the unique subunit stoichiometry prevailing in TFIID and uncover a hexameric arrangement of Tafs containing a histone fold domain in the Twin lobe.

PubMed: 30405110
DOI: 10.1038/s41467-018-07096-y

Experimental method

ELECTRON MICROSCOPY (7.1 Å)