6HPR

Crystal structure of cIAP1 RING domain bound to UbcH5B-Ub and a non-covalent Ub

6HPR の概要

• エントリーDOI: 10.2210/pdb6hpr/pdb
• 分子名称: Baculoviral IAP repeat-containing protein 2, Polyubiquitin-B, Ubiquitin-conjugating enzyme E2 D2, ... (5 entities in total)
• 機能のキーワード: ubiquitin, e3, ciap1, ubch5b, ubiquitin ligase, ligase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 42062.09

構造登録者

Patel, A.,Huang, D.T. (登録日: 2018-09-21, 公開日: 2018-12-12, 最終更新日: 2024-01-24)

主引用文献

Patel, A.,Sibbet, G.J.,Huang, D.T.
Structural insights into non-covalent ubiquitin activation of the cIAP1-UbcH5B∼ubiquitin complex.
J. Biol. Chem., 294:1240-1249, 2019

PubMed Abstract: Ubiquitin (Ub)-conjugating enzymes and Ub ligases control protein degradation and regulate many cellular processes in eukaryotes. Cellular inhibitor of apoptosis protein-1 (cIAP1) plays a central role in apoptosis and tumor necrosis factor signaling. It harbors a C-terminal RING domain that homodimerizes to recruit E2∼Ub (where ∼ denotes a thioester bond) complex to catalyze Ub transfer. Noncovalent Ub binding to the backside of the E2 Ub-conjugating enzyme UbcH5 has previously been shown to enhance RING domain activity, but the molecular basis for this enhancement is unclear. To investigate how dimeric cIAP1 RING activates E2∼Ub for Ub transfer and what role noncovalently bound Ub has in Ub transfer, here we determined the crystal structure of the cIAP1 RING dimer bound to both UbcH5B covalently linked to Ub (UbcH5B-Ub) and a noncovalent Ub to 1.7 Å resolution. The structure along with biochemical analyses revealed that the cIAP1 RING domain interacts with UbcH5B-Ub and thereby promotes the formation of a closed UbcH5B-Ub conformation that primes the thioester bond for Ub transfer. We observed that the noncovalent Ub binds to the backside of UbcH5B and abuts UbcH5B's α1β1-loop, which, in turn, stabilizes the closed UbcH5B-Ub conformation. Our results disclose the mechanism by which cIAP1 RING dimer activates UbcH5B∼Ub and indicate that noncovalent Ub binding further stabilizes the cIAP1-UbcH5B∼Ub complex in the active conformation to stimulate Ub transfer.

PubMed: 30523153
DOI: 10.1074/jbc.RA118.006045

実験手法

X-RAY DIFFRACTION (1.7 Å)