6GTF

Transient state structure of CRISPR-Cpf1 (Cas12a) I5 conformation

Summary for 6GTF

EMDB information0064
DescriptorCRISPR-associated endonuclease Cas12a, RNA (29-MER), DNA (5'-D(P*AP*TP*GP*AP*CP*TP*TP*CP*TP*CP*TP*AP*AP*CP*AP*AP*GP*CP*TP*CP*G)-3'), ... (4 entities in total)
Functional Keywordscrispr genome editing ribonucleoprotein complex, hydrolase
Biological sourceFrancisella tularensis subsp. novicida U112
Total number of polymer chains4
Total molecular weight203280.74
Authors
Montoya, G.,Mesa, P.,Stella, S. (deposition date: 2018-06-18, release date: 2018-12-19, Last modification date: 2018-12-26)
Primary citation
Stella, S.,Mesa, P.,Thomsen, J.,Paul, B.,Alcon, P.,Jensen, S.B.,Saligram, B.,Moses, M.E.,Hatzakis, N.S.,Montoya, G.
Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity.
Cell, 175:1856-1871.e21, 2018
PubMed: 30503205 (PDB entries with the same primary citation)
DOI: 10.1016/j.cell.2018.10.045
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
?

Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone100 1.3% 0.22MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures