Transient state structure of CRISPR-Cpf1 (Cas12a) I5 conformation

Summary for 6GTF

EMDB information0064
DescriptorCRISPR-associated endonuclease Cas12a, RNA (29-MER), DNA (5'-D(P*AP*TP*GP*AP*CP*TP*TP*CP*TP*CP*TP*AP*AP*CP*AP*AP*GP*CP*TP*CP*G)-3'), ... (4 entities in total)
Functional Keywordscrispr genome editing ribonucleoprotein complex, hydrolase
Biological sourceFrancisella tularensis subsp. novicida U112
Total number of polymer chains4
Total molecular weight203280.74
Montoya, G.,Mesa, P.,Stella, S. (deposition date: 2018-06-18, release date: 2018-12-19, Last modification date: 2019-12-11)
Primary citation
Stella, S.,Mesa, P.,Thomsen, J.,Paul, B.,Alcon, P.,Jensen, S.B.,Saligram, B.,Moses, M.E.,Hatzakis, N.S.,Montoya, G.
Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity.
Cell, 175:1856-1871.e21, 2018
PubMed: 30503205 (PDB entries with the same primary citation)
DOI: 10.1016/j.cell.2018.10.045
MImport into Mendeley
Experimental method

Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone100 1.3% 0.22MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures
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