6GTF

Transient state structure of CRISPR-Cpf1 (Cas12a) I5 conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CRISPR-associated endonuclease Cas12a	polymer	1329	155639.8	1	UniProt (A0Q7Q2) Pfam (PF18501) Pfam (PF18516) Pfam (PF18510) In PDB	Francisella tularensis subsp. novicida U112	CRISPR-associated endonuclease Cpf1,FnCas12a,FnCpf1
2	B	RNA (29-MER)	polymer	43	13749.1	1		Francisella tularensis subsp. novicida U112
3	C	DNA (5'-D(P*AP*TP*GP*AP*CP*TP*TP*CP*TP*CP*TP*AP*AP*CP*AP*AP*GP*CP*TP*CP*G)-3')	polymer	55	16898.8	1		Francisella tularensis subsp. novicida U112
4	D	DNA (5'-D(P*CP*GP*AP*GP*CP*TP*CP*GP*TP*TP*AP*GP*AP*GP*AP*A)-3')	polymer	55	16992.9	1		Francisella tularensis subsp. novicida U112

Sequence modifications

A: 1 - 1300 (UniProt: A0Q7Q2)

PDB	External Database	Details
Gln 1006	Glu 1006	conflict
Gly 1301	-	expression tag
Ser 1302	-	expression tag
Glu 1303	-	expression tag
Phe 1304	-	expression tag
Glu 1305	-	expression tag
Leu 1306	-	expression tag
Glu 1307	-	expression tag
Asn 1308	-	expression tag
Leu 1309	-	expression tag
Tyr 1310	-	expression tag
Phe 1311	-	expression tag
Gln 1312	-	expression tag
Gly 1313	-	expression tag
Glu 1314	-	expression tag
Leu 1315	-	expression tag
Arg 1316	-	expression tag
Arg 1317	-	expression tag
Gln 1318	-	expression tag
Ala 1319	-	expression tag
Ser 1320	-	expression tag
Ala 1321	-	expression tag
Leu 1322	-	expression tag
Glu 1323	-	expression tag
His 1324	-	expression tag
His 1325	-	expression tag
His 1326	-	expression tag
His 1327	-	expression tag
His 1328	-	expression tag
His 1329	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	203280.7
All*	Total formula weight	203280.7

*Water molecules are not included.