6GTF
Transient state structure of CRISPR-Cpf1 (Cas12a) I5 conformation
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0004530 | molecular_function | deoxyribonuclease I activity |
| A | 0006259 | biological_process | DNA metabolic process |
| A | 0016829 | molecular_function | lyase activity |
| A | 0051607 | biological_process | defense response to virus |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 3 |
| Details | ACT_SITE: For pre-crRNA processing => ECO:0000305|PubMed:28431230 |
| Chain | Residue | Details |
| A | HIS843 | |
| A | LYS852 | |
| A | LYS869 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 3 |
| Details | ACT_SITE: For DNase activity of RuvC domain => ECO:0000305|PubMed:26422227 |
| Chain | Residue | Details |
| A | ASP917 | |
| A | GLN1006 | |
| A | ASP1255 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | SITE: Binds crRNA alone and in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
| Chain | Residue | Details |
| A | THR16 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | SITE: Binds target strand DNA => ECO:0000269|PubMed:28431230 |
| Chain | Residue | Details |
| A | LYS131 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | SITE: Binds crRNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 |
| Chain | Residue | Details |
| A | THR295 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 3 |
| Details | SITE: Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 |
| Chain | Residue | Details |
| A | LYS320 | |
| A | SER334 | |
| A | LYS589 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | SITE: Caps the crRNA-target DNA heteroduplex => ECO:0000305|PubMed:28431230 |
| Chain | Residue | Details |
| A | TYR410 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | SITE: Binds DNA protospacer adjacent motif (PAM) => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
| Chain | Residue | Details |
| A | LYS613 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | SITE: Binds Target strand DNA => ECO:0000269|PubMed:28431230 |
| Chain | Residue | Details |
| A | LYS667 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | SITE: Binds PAM => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
| Chain | Residue | Details |
| A | LYS671 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | SITE: Binds Target strand DNA => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
| Chain | Residue | Details |
| A | LYS677 | |
| A | LYS823 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | SITE: Binds Target strand DNA; via amide nitrogen => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
| Chain | Residue | Details |
| A | GLY826 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | SITE: Binds crRNA => ECO:0000269|PubMed:28431230 |
| Chain | Residue | Details |
| A | ARG833 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 1 |
| Details | SITE: Stabilizes transition state for pre-crRNA processing => ECO:0000305|PubMed:28431230 |
| Chain | Residue | Details |
| A | LYS852 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 2 |
| Details | SITE: Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
| Chain | Residue | Details |
| A | LYS1026 | |
| A | THR1063 |
