6GSN
Structure of a partial yeast 48S preinitiation complex in closed conformation
This is a non-PDB format compatible entry.
Summary for 6GSN
| Entry DOI | 10.2210/pdb6gsn/pdb |
| Related | 3JAQ |
| EMDB information | 0058 3048 3049 |
| Descriptor | 18S rRNA (1798-MER), KLLA0B01562p, KLLA0A07194p, ... (51 entities in total) |
| Functional Keywords | ribosome, translation, initiation factors, 40s, eif1a, eif3, eif2, trnai, 48s pic, small ribosome subunit |
| Biological source | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) More |
| Total number of polymer chains | 47 |
| Total formula weight | 1543510.48 |
| Authors | Llacer, J.L.,Hussain, T.,Ramakrishnan, V. (deposition date: 2018-06-14, release date: 2019-06-26, Last modification date: 2024-11-13) |
| Primary citation | Llacer, J.L.,Hussain, T.,Dong, J.,Villamayor, L.,Gordiyenko, Y.,Hinnebusch, A.G. Large-scale movement of eIF3 domains during translation initiation modulate start codon selection. Nucleic Acids Res., 2021 Cited by PubMed Abstract: The eukaryotic initiation factor 3 (eIF3) complex is involved in every step of translation initiation, but there is limited understanding of its molecular functions. Here, we present a single particle electron cryomicroscopy (cryo-EM) reconstruction of yeast 48S ribosomal preinitiation complex (PIC) in an open conformation conducive to scanning, with core subunit eIF3b bound on the 40S interface near the decoding center in contact with the ternary complex eIF2·GTP·initiator tRNA. eIF3b is relocated together with eIF3i from their solvent interface locations observed in other PIC structures, with eIF3i lacking 40S contacts. Re-processing of micrographs of our previous 48S PIC in a closed state also suggests relocation of the entire eIF3b-3i-3g-3a-Cter module during the course of initiation. Genetic analysis indicates that high fidelity initiation depends on eIF3b interactions at the 40S subunit interface that promote the closed PIC conformation, or facilitate the relocation of eIF3b/eIF3i to the solvent interface, on start codon selection. PubMed: 34648019DOI: 10.1093/nar/gkab908 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (5.75 Å) |
Structure validation
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