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6FY4

Structure of human NAD(P) H:quinone oxidoreductase in complex with N-(2-bromophenyl)pyrrolidine-1-sulfonamide

Summary for 6FY4
Entry DOI10.2210/pdb6fy4/pdb
DescriptorNAD(P)H dehydrogenase [quinone] 1, FLAVIN-ADENINE DINUCLEOTIDE, N-(2-bromophenyl)pyrrolidine-1-sulfonamide (3 entities in total)
Functional Keywordsflavoprotein ligand complex quinone reduction oxidative stress drug metabolism, oxidoreductase
Biological sourceHomo sapiens (Human)
Total number of polymer chains4
Total formula weight127077.84
Authors
Gruber, K.,Hromic, A. (deposition date: 2018-03-10, release date: 2019-03-20, Last modification date: 2024-01-17)
Primary citationStrandback, E.,Lienhart, W.D.,Hromic-Jahjefendic, A.,Bourgeois, B.,Hogler, A.,Waltenstorfer, D.,Winkler, A.,Zangger, K.,Madl, T.,Gruber, K.,Macheroux, P.
A small molecule chaperone rescues the stability and activity of a cancer-associated variant of NAD(P)H:quinone oxidoreductase 1 in vitro.
Febs Lett., 594:424-438, 2020
Cited by
PubMed Abstract: NAD(P)H:quinone oxidoreductase 1 (NQO1) is a human FAD-dependent enzyme that plays a crucial role in the antioxidant defense system. A naturally occurring single-nucleotide polymorphism (NQO1*2) in the NQO1 gene leads to an amino acid substitution (P187S), which severely compromises the activity and stability of the enzyme. The NQO1*2 genotype has been linked to a higher risk for several types of cancer and poor survival rate after anthracycline-based chemotherapy. In this study, we show that a small molecular chaperone (N-(2-bromophenyl)pyrrolidine-1-sulfonamide) repopulates the native wild-type conformation. As a consequence of the stabilizing effect, the enzymatic activity of the P187S variant protein is strongly improved in the presence of the molecular chaperone in vitro.
PubMed: 31605637
DOI: 10.1002/1873-3468.13636
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.76 Å)
Structure validation

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