6FWX

Chimeric titin Z1Z2-Z1Z2 tandem (Z1212) functionalized with a GRGDS exogenous peptide from fibronectin

Summary for 6FWX

• Entry DOI: 10.2210/pdb6fwx/pdb
• Related: 1YA5 2A38 2F8V
• Descriptor: Titin,Titin,Titin, 1,2-ETHANEDIOL (2 entities in total)
• Functional Keywords: titin, z1z2, structural protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 83993.10

Authors

Mayans, O.,Fleming, J.,Hill, C. (deposition date: 2018-03-07, release date: 2019-03-27, Last modification date: 2024-01-17)

Primary citation

Hill, C.J.,Fleming, J.R.,Mousavinejad, M.,Nicholson, R.,Tzokov, S.B.,Bullough, P.A.,Bogomolovas, J.,Morgan, M.R.,Mayans, O.,Murray, P.
Self-Assembling Proteins as High-Performance Substrates for Embryonic Stem Cell Self-Renewal.
Adv. Mater. Weinheim, 31:e1807521-e1807521, 2019

PubMed Abstract: The development of extracellular matrix mimetics that imitate niche stem cell microenvironments and support cell growth for technological applications is intensely pursued. Specifically, mimetics are sought that can enact control over the self-renewal and directed differentiation of human pluripotent stem cells (hPSCs) for clinical use. Despite considerable progress in the field, a major impediment to the clinical translation of hPSCs is the difficulty and high cost of large-scale cell production under xeno-free culture conditions using current matrices. Here, a bioactive, recombinant, protein-based polymer, termed ZT , is presented that closely mimics human plasma fibronectin and serves as an economical, xeno-free, biodegradable, and functionally adaptable cell substrate. The ZT substrate supports with high performance the propagation and long-term self-renewal of human embryonic stem cells while preserving their pluripotency. The ZT polymer can, therefore, be proposed as an efficient and affordable replacement for fibronectin in clinical grade cell culturing. Further, it can be postulated that the ZT polymer has significant engineering potential for further orthogonal functionalization in complex cell applications.

PubMed: 30866118
DOI: 10.1002/adma.201807521

Experimental method

X-RAY DIFFRACTION (3 Å)