6FB6

Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with an altered version of its target DNA (Haemoglobin beta subunit gene) at 5NNN region in the presence of Manganese

Summary for 6FB6

• Entry DOI: 10.2210/pdb6fb6/pdb
• Descriptor: I-CreI monomer A, I-CreI monomer B, DNA (5'-D(*TP*CP*AP*GP*AP*CP*TP*TP*GP*TP*CP*CP*AP*C)-3'), ... (8 entities in total)
• Functional Keywords: chlamydomonas reinhardtii, dna binding protein
• Biological source: Chlamydomonas reinhardtii; More
• Total number of polymer chains: 6
• Total formula weight: 49941.79

Authors

Molina, R.,Prieto, J. (deposition date: 2017-12-18, release date: 2018-07-18, Last modification date: 2024-01-17)

• Primary citation: Prieto, J.,Redondo, P.,Lopez-Mendez, B.,D'Abramo, M.,Merino, N.,Blanco, F.J.,Duchateau, P.,Montoya, G.,Molina, R.; Understanding the indirect DNA read-out specificity of I-CreI Meganuclease.; Sci Rep, 8:10286-10286, 2018; PubMed: 29980759; DOI: 10.1038/s41598-018-28599-0

Experimental method

X-RAY DIFFRACTION (2.6 Å)