6FB6
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with an altered version of its target DNA (Haemoglobin beta subunit gene) at 5NNN region in the presence of Manganese
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | I-CreI monomer A | polymer | 153 | 17528.2 | 1 | Pfam (PF00961) UniProt (by SIFTS) (P05725) In PDB | Chlamydomonas reinhardtii | |
2 | B | I-CreI monomer B | polymer | 154 | 17583.2 | 1 | Pfam (PF00961) UniProt (by SIFTS) (P05725) In PDB | Chlamydomonas reinhardtii | |
3 | D | DNA (5'-D(*TP*CP*AP*GP*AP*CP*TP*TP*GP*TP*CP*CP*AP*C)-3') | polymer | 14 | 4215.8 | 1 | synthetic construct | ||
4 | F | DNA (5'-D(*TP*CP*TP*GP*AP*CP*TP*CP*CP*TP*GP*TP*GP*G)-3') | polymer | 14 | 4262.8 | 1 | synthetic construct | ||
5 | E | DNA (5'-D(P*GP*GP*GP*AP*GP*TP*CP*AP*GP*A)-3') | polymer | 10 | 3134.1 | 1 | synthetic construct | ||
6 | G | DNA (5'-D(P*AP*CP*AP*AP*GP*TP*CP*TP*GP*A)-3') | polymer | 10 | 3053.0 | 1 | synthetic construct | ||
7 | A | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) | |||
8 | water | water | 18.0 | 11 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 49777.0 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 164.8 | |
All* | Total formula weight | 49941.8 |