6FB6
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with an altered version of its target DNA (Haemoglobin beta subunit gene) at 5NNN region in the presence of Manganese
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | I-CreI monomer A | polymer | 153 | 17528.2 | 1 | Pfam (PF00961) UniProt (by SIFTS) (P05725) | Chlamydomonas reinhardtii | |
| 2 | B (B) | I-CreI monomer B | polymer | 154 | 17583.2 | 1 | Pfam (PF00961) UniProt (by SIFTS) (P05725) | Chlamydomonas reinhardtii | |
| 3 | C (D) | DNA (5'-D(*TP*CP*AP*GP*AP*CP*TP*TP*GP*TP*CP*CP*AP*C)-3') | polymer | 14 | 4215.8 | 1 | synthetic construct | ||
| 4 | D (F) | DNA (5'-D(*TP*CP*TP*GP*AP*CP*TP*CP*CP*TP*GP*TP*GP*G)-3') | polymer | 14 | 4262.8 | 1 | synthetic construct | ||
| 5 | E (E) | DNA (5'-D(P*GP*GP*GP*AP*GP*TP*CP*AP*GP*A)-3') | polymer | 10 | 3134.1 | 1 | synthetic construct | ||
| 6 | F (G) | DNA (5'-D(P*AP*CP*AP*AP*GP*TP*CP*TP*GP*A)-3') | polymer | 10 | 3053.0 | 1 | synthetic construct | ||
| 7 | G, H, I (A) | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) | |||
| 8 | J, K, L, M (A, B, F, G) | water | water | 18.0 | 11 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 49777.0 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 164.8 | |
| All* | Total formula weight | 49941.8 |
