6FB6
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with an altered version of its target DNA (Haemoglobin beta subunit gene) at 5NNN region in the presence of Manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.6 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.176, 67.987, 83.044 |
| Unit cell angles | 90.00, 96.45, 90.00 |
Refinement procedure
| Resolution | 44.890 - 2.600 |
| R-factor | 0.1978 |
| Rwork | 0.193 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9z |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.780 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.890 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.110 | 0.540 |
| Number of reflections | 28700 | |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 96.1 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol |
