6FB6

Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with an altered version of its target DNA (Haemoglobin beta subunit gene) at 5NNN region in the presence of Manganese

Summary for 6FB6

DescriptorI-CreI monomer A, I-CreI monomer B, DNA (5'-D(*TP*CP*AP*GP*AP*CP*TP*TP*GP*TP*CP*CP*AP*C)-3'), ... (8 entities in total)
Functional Keywordschlamydomonas reinhardtii, dna binding protein
Biological sourceChlamydomonas reinhardtii
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Total number of polymer chains6
Total molecular weight49941.79
Authors
Molina, R.,Prieto, J. (deposition date: 2017-12-18, release date: 2018-07-18)
Primary citation
Prieto, J.,Redondo, P.,Lopez-Mendez, B.,D'Abramo, M.,Merino, N.,Blanco, F.J.,Duchateau, P.,Montoya, G.,Molina, R.
Understanding the indirect DNA read-out specificity of I-CreI Meganuclease.
Sci Rep, 8:10286-10286, 2018
PubMed: 29980759 (PDB entries with the same primary citation)
DOI: 10.1038/s41598-018-28599-0
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.6 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.20140.3%1.4%3.4%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution