6EVV

X-ray structure of the complex between human alpha thrombin and NU172, a duplex/quadruplex 26-mer DNA aptamer, in the presence of potassium ions.

6EVV の概要

• エントリーDOI: 10.2210/pdb6evv/pdb
• 関連するBIRD辞書のPRD_ID: PRD_000020
• 分子名称: Prothrombin, NU172, DNA (26-MER), N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: thrombin-dna, g-quadruplex, aptamer, duplex/quadruplex, hydrolase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 44224.55

構造登録者

Troisi, R.,Russo Krauss, I.,Sica, F. (登録日: 2017-11-02, 公開日: 2018-10-24, 最終更新日: 2024-11-06)

主引用文献

Troisi, R.,Napolitano, V.,Spiridonova, V.,Russo Krauss, I.,Sica, F.
Several structural motifs cooperate in determining the highly effective anti-thrombin activity of NU172 aptamer.
Nucleic Acids Res., 46:12177-12185, 2018

PubMed Abstract: Despite aptamers are very promising alternative to antibodies, very few of them are under clinical trials or are used as drugs. Among them, NU172 is currently in Phase II as anticoagulant in heart disease treatments. It inhibits thrombin activity much more effectively than TBA, the best-known thrombin binding aptamer. The crystal structure of thrombin-NU172 complex reveals a bimodular duplex/quadruplex architecture for the aptamer, which binds thrombin exosite I through a highly complementary surface involving all three loops of the G-quadruplex module. Although the duplex domain does not interact directly with thrombin, the features of the duplex/quadruplex junction and the solution data on two newly designed NU172 mutants indicate that the duplex moiety is important for the optimization of the protein-ligand interaction and for the inhibition of the enzyme activity. Our work discloses the structural features determining the inhibition of thrombin by NU172 and put the basis for the design of mutants with improved properties.

PubMed: 30357392
DOI: 10.1093/nar/gky990

実験手法

X-RAY DIFFRACTION (2.5 Å)