6EDW

Crystal structure of Mycobacterium tuberculosis ICL2 in the apo form

6EDW の概要

• エントリーDOI: 10.2210/pdb6edw/pdb
• 関連するPDBエントリー: 6EDZ 6EE1
• 分子名称: Isocitrate lyase 2, GLYCEROL, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: lyase
• 由来する生物種: Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 351094.51

構造登録者

Bashiri, G.,Bhusal, R.,Leung, I. (登録日: 2018-08-12, 公開日: 2019-08-14, 最終更新日: 2019-10-23)

主引用文献

Bhusal, R.P.,Jiao, W.,Kwai, B.X.C.,Reynisson, J.,Collins, A.J.,Sperry, J.,Bashiri, G.,Leung, I.K.H.
Acetyl-CoA-mediated activation of Mycobacterium tuberculosis isocitrate lyase 2.
Nat Commun, 10:4639-4639, 2019

PubMed Abstract: Isocitrate lyase is important for lipid utilisation by Mycobacterium tuberculosis but its ICL2 isoform is poorly understood. Here we report that binding of the lipid metabolites acetyl-CoA or propionyl-CoA to ICL2 induces a striking structural rearrangement, substantially increasing isocitrate lyase and methylisocitrate lyase activities. Thus, ICL2 plays a pivotal role regulating carbon flux between the tricarboxylic acid (TCA) cycle, glyoxylate shunt and methylcitrate cycle at high lipid concentrations, a mechanism essential for bacterial growth and virulence.

PubMed: 31604954
DOI: 10.1038/s41467-019-12614-7

実験手法

X-RAY DIFFRACTION (1.8 Å)