6DJT
Structure of TYW1 with a lysine-pyruvate adduct bound
Summary for 6DJT
| Entry DOI | 10.2210/pdb6djt/pdb |
| Descriptor | S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase, IRON/SULFUR CLUSTER, GLYCEROL, ... (6 entities in total) |
| Functional Keywords | s-adenosylmethionine radical enzymes, trna biosynthetic enzyme, metal binding protein |
| Biological source | Methanocaldococcus jannaschii (Methanococcus jannaschii) |
| Total number of polymer chains | 1 |
| Total formula weight | 40520.74 |
| Authors | Grell, T.A.J.,Drennan, C.L. (deposition date: 2018-05-26, release date: 2018-06-13, Last modification date: 2023-11-29) |
| Primary citation | Grell, T.A.J.,Young, A.P.,Drennan, C.L.,Bandarian, V. Biochemical and Structural Characterization of a Schiff Base in the Radical-Mediated Biosynthesis of 4-Demethylwyosine by TYW1. J. Am. Chem. Soc., 140:6842-6852, 2018 Cited by PubMed Abstract: TYW1 is a radical S-adenosyl-l-methionine (SAM) enzyme that catalyzes the condensation of pyruvate and N-methylguanosine to form the posttranscriptional modification, 4-demethylwyosine, in situ on transfer RNA (tRNA). Two mechanisms have been proposed for this transformation, with one of the possible mechanisms invoking a Schiff base intermediate formed between a conserved lysine residue and pyruvate. Utilizing a combination of mass spectrometry and X-ray crystallography, we have obtained evidence to support the formation of a Schiff base lysine adduct in TYW1. When C labeled pyruvate is used, the mass shift of the adduct matches that of the labeled pyruvate, indicating that pyruvate is the source of the adduct. Furthermore, a crystal structure of TYW1 provides visualization of the Schiff base lysine-pyruvate adduct, which is positioned directly adjacent to the auxiliary [4Fe-4S] cluster. The adduct coordinates the unique iron of the auxiliary cluster through the lysine nitrogen and a carboxylate oxygen, reminiscent of how the radical SAM [4Fe-4S] cluster is coordinated by SAM. The structure provides insight into the binding site for tRNA and further suggests how radical SAM chemistry can be combined with Schiff base chemistry for RNA modification. PubMed: 29792696DOI: 10.1021/jacs.8b01493 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.64 Å) |
Structure validation
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